Summary Research scientists at National Jewish Health have developed a C57BL/10 targeted mutation mouse strain that expresses defective T cell receptors and spontaneously develop keratitis. Genes from mice of the C57BL/6 background, which carry existing genetically engineered mutations that prevent the development of?d or aß T cells, were transferred onto the C57BL/10 background.
Animal model for keratitis where the development is spontaneous
A model to test compounds developed to treat keratitis or influence angiogenesis
Advantages of Invention B10.TCR d -/- and B10-TCR ß-/- female mice show a high frequency of spontaneous keratitis. The B10.TCR ß/d -/- females have a lower incidence of spontaneous keratitis.
State of Development To establish new C57BL/10 background mouse strains, ten or more backcrosses were carried out. The B10.TCR d -/- mice have defective T cell receptor-Cd gene; the B10-TCR ß-/- mice have defective T cell receptor Cß gene. These mice were intercrossed to establish the B10.TCR ß/d -/- strain.
B10.TCR d-/- and B10.TCR b-/- mice develop a spontaneous eye disease at a high frequency. Left - normal C57BL/10 eye. Right - B10.TCR d-/- mice showing corneal opacity. Note the central thickening.
Histological sections (H&E stained) showing that the corneal opacity in these mice is due to keratitis. 1 and 2, normal C57BL/10 eye shown for comparison. Panels 2-6 are close-ups of the corneal area only, and examples of increasing severity are shown. Note the inflammatory infiltrates in the corneal stroma, also the presence of blood vessels in the stroma, and the occasional thickening of the corneal epithelium.
O'brien, R. L., M. A. Taylor, J. Hartley, T. Nuhsbaum, S. Dugan, K. Lahmers, M. K. Aydintug, J. M. Wands, C. L. Roark, and W. K. Born. "Protective Role of T Cells in Spontaneous Ocular Inflammation." Investigative Ophthalmology & Visual Science 50.7 (2009): 3266-274. PMID: 19151391