Lilliana Radoshevich, PhD

Special Interests

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Research in the Radoshevich laboratory is focused on host responses to the cytosolic intracellular bacterial pathogens: Listeria and Francisella. More specifically, we explore changes in the post-translational landscape of the cell following stress or infection. Ubiquitin-like modifications (UBLs) are rapid, reversible and can profoundly alter cell fate and function. Intriguingly, the majority of UBLs are involved in the cellular response to stress, in particular the response to infection and autophagy. We take an interdisciplinary approach combining cutting-edge proteomics with genome editing, biochemistry, cell biology, and in vivo infection models to determine fundamental properties and modes of action of understudied ubiquitin-like modifications. We aim to address the central question of how the cell responds to stress by decoding novel networks of covalent protein complexes. We also aim to decipher which virulence factors hijack and thwart cellular stress responses to illuminate how intracellular pathogens survive and replicate in the host. Most importantly the enzymatic systems that activate, conjugate and ligate UBLs to their protein substrates are promising targets for therapeutic intervention in a number of important human diseases such as cancer, neurodegenerative and autoimmune disorders.

Education

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Education

2005 - 2011

University of California, San Francisco, Biomedical Sciences

2000 - 2004

Grinnell College, Biology and French

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Fellowship

2011 - 2017

Institut Pasteur, Host-Pathogen Interactions

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Awards & Recognition

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Member of Colorado Biological Mass Spectrometry Society Board
Inaugural Cohort of Stead Scholars, Carver College of Medicine
Human Frontiers Science Foundation Fellowship
European Molecular Biology Organization Fellowship
Emile Roux Fellowship, Institut Pasteur
Phi Beta Kappa, Elizabeth B. Reed Scholarship
Genentech/Sandler Graduate Fellowship, UCSF
Graduate Dean’s Health Science Fellowship, UCSF
 

Professional Memberships

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American Society for Cell Biology, Colorado Biological Mass Spectrometry Society, International Society for Tularemia, Human Proteomics Society 

Publications

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Zhang Y, Patterson J, Ripley B, Rojas-Chávez RA, Wei O, Coloma Ciudad R, Sturtz M, Upton E, Luhmann E, Schwery N, Anthony S, Goeken A, Moninger T, Harty J, Klingelhutz A, Lundberg E, Meyerholz DK, Stipp C, Manicassamy B, Guerra S, and Radoshevich L. Listeria monocytogenes hijacks ISG15-modification of the Arp2/3 complex in conditions of enhanced ISGylation. In Press

Thery, F, Martina L, Asselman C, Zhang Y, Vessely M, Repo H, Sedeyn K, Moschonas, GD, Bredow, C, Teo QW, Zhang J, Leandro K, Eggermont D, De Sutter D, Boucher K, Hochepied T, Festjens N, Callewaert N, Saelens X, Dermaut B, Knobeloch KP, Beling A, Sanyal S, Radoshevich L*, Eyckerman S*, and Impens F*. Ring finger protein 213 assembles into a sensor for ISGylated proteins with antimicrobial activity. Nat Commun Oct 1 2020 12(1): 5772. * co-corresponding author

Zhang Y, Thery F, Wu NC, Luhmann EK, Dussurget O, Foecke M, Bredow C, Jiménez-Fernández D, Leandro K, Beling A, Knobeloch KP, Impens F, Cossart P, Radoshevich L. The in vivo ISGylome links ISG15 to metabolic pathways and autophagy upon Listeria monocytogenes infection. Nat Commun. 2019 Nov 26;10(1):5383.

Radoshevich L*, Impens F, Ribet D, Quereda JJ, Tham TN, Nahori MA, Bierne H, Dussurget O, PizarroCerdá J, Knobeloch, KP, and Cossart P*. 2015. ISG15 counteracts Listeria monocytogenes infection. eLife. 2015 Aug 11; 4.
* co-corresponding author

Radoshevich L, Murrow L, Fernandez E, Chen N, Roy S, Fung C and Debnath J. ATG12 conjugation to ATG3 regulates mitochondrial homeostasis and cell death. Cell. 2010.142(4), 590-600.

 

Academic Affiliations

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Affiliate University of Colorado Anschutz, Department of Immunology and Microbiology

Teaching & Professional Positions

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Assistant Professor, Department of Microbiology and Immunology, University of Iowa 2017-2023