My lab studies transcriptional and epigenetic regulation of genes that control immune effector cell development and regulate cytokine and chemokine gene expression. We investigate how super-enhancers and their associated transcription factors integrate signals triggered by various external stimuli, such as antigenic stimulation, infection, cytokine and metabolic products, to generate gene transcription outputs. We also determine the contribution of genetic variants to enhancer activity, gene expression and disease severity. We utilize various cutting-edge technologies, including next-generation sequencing, bioinformatics, enhancer library and CRISPR to address significant research problems in inflammation and allergic immune responses.
Li YL, Gao JF, Zhao DZ, Guan XY, Morris SC, Finkelman FD, Huang H. The GC box at the proximal enhancer of the Hdc gene is critical for Hdc gene transcription and histamine-mediated anaphylaxis. (BioRxiv 495950) [Preprint], available from: https://doi.org/10.1101/2022.06.13.495950. J Allergy Clin Immunol, published online Feb. 23, 2023, 10.1016/j.jaci.2023.01.031.
Li, Y., Gao, J. F., Kamran, M. Harmacek, L., Danhorn, T., Leach, S. M., O'Connor, B. P., Hagman, J. R., Huang H. GATA2 regulates mast cell identity and responsiveness to antigenic stimulation by promoting chromatin remodeling at super-enhancers. Nat Commun 12, 494 (2021). https://doi.org/10.1038/s41467-020-20766-0
Kamran, M.*, Liang, J.Y., 2*, Liu, B., Li, Y., Gao, J.F. Keating, A., Mohamed, F., Dai S.D., Reinhardt, R., Yang, J., Wu, Z.D., Huang H. The Clusters of Transcription Factors NFATC2, STAT5, GATA2, AP1, RUNX1 and EGR2 Binding Sites at the Induced Il13 Enhancers Mediate Il13 Gene Transcription in Response to Antigenic Stimulation. J Immunol, Nov, 13, 2020, 10.4049/jimmunol.2000985.
Li Y, Qi X, Liu B, and Huang H. The STAT5-GATA2 Pathway Is Critical in Basophil and Mast Cell Differentiation and Maintenance. J Immunol. 2015, 194: 4328-38. (Also see Editorial in “In This Issue”)
Qi X, L. Chaves, Y.H. Zhuang, Y. Chen, D. Wang, J. Chahon, B. Graham, K. Ohmori, and Huang H. Antagonistic regulation by transcription factors C/EBPa and MITF specifies basophil and mast cell fates. Immunity, 2013, 39, 97-110.