Kappler Marrack Research Lab

Protocols

B Cell Fusion and T Cell Fusion Protocols

• B cell Fusion
• T cell Fusion
• How to prime T cells in vitro and make T cell hybridoma
• Freezing and Thawing T or B cell Hybridomas

Baculovirus Culture Products

• Baculovirus Cloning
• CoTransfection of Baculovirus
• Produce Hi5Protein in Spinners
• Virus Stocks SF9

Labeling Procedures

• Biotinylation of Bir A-peptide tagged proteins

Cell Staining Procedures

• Staining T cell hybridomas
• Staining ex vivo samples with IAg7 Tetramers

Media Recipes

• Click's Medium
• Kappler-Marrack Complete Tumor Medium
• Tumor Cocktail for DMEM

PCR for Mouse Screens

• Genomic DNA from mouse ear punches
• Mouse Typing From Ears
• lpr/lpr
• RAG-2 KO
• TNFRI(p55)
• TNFRI(p75)