Neutrophil Adherence Markers (includes CD11a, CD11b, CD11c and CD18)
Test Code
ADHM
Description
Collect
Draw one green top tube (Sodium or Lithium Heparin) top tube.
Specimen Preparation
Do NOT centrifuge! Keep at room temperature (18-22°C).
Preferred volume: 4 mL
Pediatric Collection
Minimum volume: 2 mL blood
Unacceptable Conditions
Frozen, clotted, hemolyzed or centrifuged specimens. Anticoagulants other than heparin. Specimens >24 hrs old.
Storage Transport Temp
Maintain and transport specimens at room temperature (18-22°C).
Ship specimens Priority Overnight via Fed Ex in container sufficiently insulated to avoid temperature extremes (<10°C and >37°C).
Stability
24 hours at room temperature (18-22°C)
Notes
Samples should be received within 24 hours of draw. Samples are accepted Monday through noon on Friday.
Reference Interval
- CD11a: >29 Mean Fluorescence Intensity (MFI)
- CD11b: >154 MFI, CD11c: >113 MFI, CD18: >192 MFI
Interpretation
LAD-1 is an autosomal recessive disorder caused by mutations in CD18, the common chain of the beta-2 integrin family. The beta-2 integrin family is comprised of LFA-1 (CD11a/CD18), Mac-1/CR3 (CD11b/CD18) and p150/95 (CD11c/CD18). Defects in CD18 therefore affect the expression of CD11a, b and c. Reduction or loss of surface expression of the beta-2 integrins supports the diagnosis of LAD-1.
CPT Code
88184, 88185 X 3