Memory T Cells
Test Code
4RARO
Description
Collect
Draw one green top tube (Sodium or Lithium Heparin) top tube.
Specimen Preparation
Do not centrifuge! Keep at room temp (18-22°C).
Preferred volume: 4 mL
Pediatric Collection
Minimum volume: 1 mL blood
Unacceptable Conditions
Frozen, clotted, hemolyzed or centrifuged specimens. Anticoagulants other than heparin. Specimens >24 hrs old.
Storage Transport Temp
Maintain and transport specimens at room temperature (18-22°C).
Ship specimens Priority Overnight via Fed Ex in container sufficiently insulated to avoid temperature extremes (<10°C and >37°C).
Stability
24 hours at room temperature (18-22°C)
Notes
Samples must arrive within 24 hours of draw and are accepted Monday through noon on Friday.
Reference Interval
| Age Range | (Naïve T cells) CD4+CD45RA+ (% of CD4) | (Memory T cells) CD4+CD45RO+ (% of CD4) |
| 0-3 months | 64-95 | 2-22 |
| 3-6 months | 77-94 | 3-16 |
| 6 months-1 year | 64-93 | 5-18 |
| 1 year – 2 years | 63-91 | 7-20 |
| 2 years – 6 years | 53-86 | 9-26 |
| 6 years – 12 years | 46-77 | 13-30 |
| 12 years – 18 years | 33-66 | 18-38 |
| > 18 years | 32-73 | 29-63 |
Interpretation
This test is useful for the analysis of naïve and memory T cells and assists in the evaluation of general immune competance, immune deficiency states such as the Di-George syndrome as well as immune reconstituition.
CPT Code
88184, 88185 x 3