Advanced Diagnostic Laboratories

Collect

Draw one green top (sodium or lithium heparin) or lavender top (EDTA) vacutainer tube.
Yellow top (ACD solution A or B) tubes are also acceptable but tube must be appropriately filled (because of liquid anticoagulant) to preserve sample integrity.

Specimen Preparation

Do not centrifuge! Keep at room temp (18-22°C).

Preferred volume: 4 mL

Pediatric Collection

Minimum volume: 1 mL blood

Unacceptable Conditions

Frozen, clotted, hemolyzed, spun down, citrate anticoagulant (blue top)

Storage Transport Temp

Maintain and transport specimens at room temperature (18-22°C).
Ship specimens Priority Overnight via Fed Ex in container sufficiently insulated to avoid temperature extremes (<10°C and >37°C).

Stability

48 hours at room temperature (18-22°C).

Notes

CBC with Differential results from within 3 days of draw must also be provided or a lavender top included with required specimen to run CBC at ADx.
Samples are accepted Monday through noon on Friday.

Performed

Tue-Fri

Methodology

Flow Cytometry

Reported

2-4 days

Department

Immunology Lab - Phenotyping Assays

Synonyms

Total T cells and subsets;Flow Cytometry (T Cell Enumeration);CD3SB

Related Tests

TBSBS, CD48, CD4M, NKMK

Reference Interval

Lymphocyte Subset Normal Range Chart

Interpretation

This panel is useful for global immune assessement of total T cells and Tcell subset percentages and numbers.

CPT Code

86359, 86360