Human Live Cell Core

Samples and Methods

De-identified Human Samples for Research

• Epithelial Cells from Bronchial Brushings (asthma, COPD, controls, …)
• Epithelial Cells from Nasal Brushings (asthma, COPD, controls, …)
• Alveolar Macrophages previously isolated from Donor Lungs (smokers and non-smokers)
• BAL Fluid and Cell Pellet in RNA Lysis Buffer
• Tracheobronchial Epithelial Cells from Donor Lungs
  • Cells for Culture
  • In RNA Lysis Buffer
  • In Western Lysis Buffer
  • Cells for any other purpose
• Lung Tissue from Donors
  • In Paraffin Blocks for Histology
  • In RNA Lysis Buffer
  • In Western Lysis Buffer
  • In RNA Later
• Distal Airway Epithelia Cells from Donor Lungs

Technical Services Offered through the Cell Core

• Culture of airway epithelial cells in submerged and air-liquid interface (ALI)
• Human precision-cut lung slices
• Human macrophage culture
• Lentivirus-mediated gene over-expression or knock-down

Banked Human Airway Epithelial Cells Available for Your Research

Tracheobronchial Epithelial Cells from Donors

Small Airway Epithelial Cells from Donors

Bronchial Epithelial Cells from Clinical Bronchoscopy and Bronchial Brushing

Nasal Epithelial Cells

How Do I Receive Samples?

1. Request Recipient Agreement and Resources Agreement forms from Niccolette Schaunaman and return both completed forms to her (No IRB protocol needed)
2. Approval within 48 hours. Niccolette Schaunaman will contact about receiving samples
3. Receive de-identified samples from Cell Core with basic demographic information

For more demographic and clinical information about samples, if needed Contact RICC-datarequests@NJHEALTH.ORG