Trikafta Level
Test Code
PKTRX
Description
This test is a high performance liquid chromatography drug monitoring assay that detects levels of elexacaftor, tezacaftor, ivacaftor and two metabolites (M1-IVA, M6-IVA) from a clinical serum specimen. The test is intended to enhance the precision and effectiveness of Trikafta therapy by tailoring the treatment to individual patients, optimizing dosages, mitigating side effects, monitoring treatment response, and offering valuable data for clinical decision-making.
Collect
Collect blood in an 8-10 mL plain red top tube.
An 8-10 mL green top tube is also acceptable for this assay, but not preferred.
Specimen Preparation
Allow to clot for 30 minutes, separate serum from cells immediately by centrifugation and aliquot into a labeled polypropylene or similar plastic tube. Use a separate tube for each test ordered. Allow room for expansion of sample inside tube.
Preferred volume: 2 mL of serum (or plasma).
Minimum volume: 0.25 mL serum (or plasma).
Pediatric Collection
If minimum volume cannot be obtained, contact Laboratory.
Unacceptable Conditions
Severe hemolysis, thawed samples for greater than 24 hours
Storage Transport Temp
Fully complete PK requisition, including drug dose amount, frequency, method, and date and time of last dose prior to draw.
Ship samples via overnight delivery on >3 lbs of dry ice.
Stability
Freeze at -70C if possible, but at minimum -20C. Up to 24 hours at room temperature.
Notes
Ship samples to be received Monday through Friday. Do not ship on Friday or Saturday. For shipping and detailed collection instructions, click here.
Performed
Weekdays
Methodology
HPLC-UV
Reported
Within 10 Business days
Department
Pharmacokinetics LabSynonyms
Kaftrio (Trikafta Level)
Reference Interval
By Report
CPT Code
80299