Vitamin D Total
Test Code
VITDT
Description
Collect
Plain red top or SST tube.
Specimen Preparation
Onsite [NJH]: Transport to lab within 1 hour.
Offsite: Separate serum from cells within 2 hours by centrifuge and aliquot into a labeled polypropylene or similar plastic tube.
Preferred volume: 1 mL serum
Offsite: Separate serum from cells within 2 hours by centrifuge and aliquot into a labeled polypropylene or similar plastic tube.
Preferred volume: 1 mL serum
Pediatric Collection
Minimum volume: 0.3mL serum
Unacceptable Conditions
Grossly lipemic or hemolyzed specimens, more than three freeze/thaw cycles
Storage Transport Temp
Offsite: Send frozen serum Priority Overnight via FedEx and in a well insulated container on dry ice.
Stability
Stability after cell separation:
72 hours at RT (15-25°C)
12 days refrigerated at 2-8°C
Indefinitely if frozen at ≤ -20°C
Performed
Mon-Fri
Methodology
Chemiluminescent Magnetic Immunoassay (CLIA)
Reported
4 hour routine, 1 hour STAT
Department
Core LabSynonyms
Vit D;Vitamin D;Ergocalciferol;cholecalciferol;Access 25(OH) Vitamin D Total;Rickets;VITDT
Reference Interval
Deficient: Less than 20 ng/dL
Insufficient: 20-29 ng/dL
Sufficient: 30-100 ng/dL
Upper Safety Limit: Greater than 100 ng/dL
CPT Code
82306