Cytokine Panel B
Test Code
THPB
Description
Cytokines are low molecular-weight proteins with numerous biological activities that are critical for immune defense, and immune surveillance. In general, Th1-type cytokines produce pro-inflammatory immune responses for killing intracellular infections such as viruses and bacteria. In contrast, Th2-type cytokines promote antibody production, eosinophil activation, and anti-inflammatory responses. Dysregulated cytokine production can lead to tissue damage, immune dysfunction and disease progression.Collect
Plasma from a lavender top (EDTA) tube is the only acceptable sample type. Mix sample thoroughly. Keep tubes cold on wet ice or with a cold pack after collection.
Patient Prep
Within 30 minutes of venipuncture, centrifuge the collection tube at 1600 (±200) x g (rcf) at 4°C (± 2) for 15 (± 1) minutes. Transfer the cell-free plasma to a clean tube and immediately store samples on dry ice or at -60°C or below in an ultra-low freezer.Preferred volume: 1 mL
Ambient temperature for centrifugation is acceptable provided that the time between collection and storage is minimized (approximately 30 minutes).
Pediatric Collection
Minimum volume: 0.5 mL plasma, frozenUnacceptable Conditions
Thawed specimen or specimens stored at room temperature prior to shipment.Storage Transport Temp
Send frozen plasma Priority Overnight via FedEx and in a well insulated container on dry ice.
Stability
-60°C or below in an ultra-low freezer - up to 1 yearNotes
This panel includes the following tests:
Interleukin 2 (aka LIL2)
Interleukin 4 (aka LIL4)
Interleukin 5 (aka LIL5)
Interleukin 10 (aka LIL10)
Interferon Gamma (aka LINFG)
Tumor Necrosis Factor alpha (aka LTNFA)
Performed
Monday - Friday
Methodology
Luminex bead based assay
Turnaround Time
up to 4 weeks
Department
Complement Lab - Cytokines
Synonyms
Complement (TH1/TH2 Panel B), Cytokine (TH1/TH2 Panel B), Cytokines (TH1/TH2 Panel B), THPNLB
Study Offerings
CAP/CLIA
Reference Interval
Interleukin 2 (IL-2): 0.00 – 1.59 pg/mLInterleukin 4 (IL-4): 0.00 – 2.18 pg/mL
Interleukin 5 (IL-5): 0.00 – 9.02 pg/mL
Interleukin 10 (IL-10): 0.00 -14.39 pg/mL
Tumor Necrosis Factor alpha (TNFA): 1.27 – 46.46 pg/mL
Interferon gamma (INFG): 0.00 - 10.74 pg/mL
Interpretation
Elevated cytokine levels could be indicative of an immune responseCPT Code
83520 x 6
New York Approved
No: Run with Waiver
FDA
LDT