Advanced Diagnostic Laboratories

Cytokine Panel B

Test Code

THPB

Description

Cytokines are low molecular-weight proteins with numerous biological activities that are critical for immune defense, and immune surveillance. In general, Th1-type cytokines produce pro-inflammatory immune responses for killing intracellular infections such as viruses and bacteria. In contrast, Th2-type cytokines promote antibody production, eosinophil activation, and anti-inflammatory responses. Dysregulated cytokine production can lead to tissue damage, immune dysfunction and disease progression.

Collect

Plasma from a lavender top (EDTA) tube is the only acceptable sample type. Mix sample thoroughly. Keep tubes cold on wet ice or with a cold pack after collection.

Patient Prep

Within 30 minutes of venipuncture, centrifuge the collection tube at 1600 (±200) x g (rcf) at 4°C (± 2) for 15 (± 1) minutes. Transfer the cell-free plasma to a clean tube and immediately store samples on dry ice or at -60°C or below in an ultra-low freezer.

Preferred volume: 1 mL

Ambient temperature for centrifugation is acceptable provided that the time between collection and storage is minimized (approximately 30 minutes).

Pediatric Collection

Minimum volume: 0.5 mL plasma, frozen

Unacceptable Conditions

Thawed specimen or specimens stored at room temperature prior to shipment.

Storage Transport Temp

Send frozen plasma Priority Overnight via FedEx and in a well insulated container on dry ice.

Stability

-60°C or below in an ultra-low freezer - up to 1 year

Notes

This panel includes the following tests:

Interleukin 2 (aka LIL2)
Interleukin 4 (aka LIL4)
Interleukin 5 (aka LIL5)
Interleukin 10 (aka LIL10)
Interferon Gamma (aka LINFG)
Tumor Necrosis Factor alpha (aka LTNFA)

Additional testing time may be needed for less than perfect samples or confirmatory/reflex testing.

Performed

Monday - Friday

Methodology

Luminex bead based assay

Turnaround Time

up to 4 weeks

Department

Complement Lab - Cytokines

Synonyms

Complement (TH1/TH2 Panel B), Cytokine (TH1/TH2 Panel B), Cytokines (TH1/TH2 Panel B), THPNLB

Study Offerings

CAP/CLIA

Reference Interval

Interleukin 2 (IL-2): 0.00 – 1.59 pg/mL
Interleukin 4 (IL-4): 0.00 – 2.18 pg/mL
Interleukin 5 (IL-5): 0.00 – 9.02 pg/mL
Interleukin 10 (IL-10): 0.00 -14.39 pg/mL
Tumor Necrosis Factor alpha (TNFA): 1.27 – 46.46 pg/mL
Interferon gamma (INFG): 0.00 - 10.74 pg/mL

Interpretation

Elevated cytokine levels could be indicative of an immune response