Advanced Diagnostic Laboratories

TH1/TH2 Panel A

Test Code

THPA

Description

Cytokines are low molecular-weight proteins with numerous biological activities that are critical for immune defense, and immune surveillance. In general, Th1-type cytokines produce pro-inflammatory immune responses for killing intracellular infections such as viruses and bacteria. In contrast, Th2-type cytokines promote antibody production, eosinophil activation, and anti-inflammatory responses. Dysregulated cytokine production can lead to tissue damage, immune dysfunction, and disease progression.

Collect

Plasma from a lavender top (K2- EDTA) tube is the only acceptable sample type. The EDTA collection tube must be completely filled with blood to ensure the correct blood to anticoagulant ratio. Invert tube several times for thorough mixing of blood anticoagulants. Keep the EDTA tube filled with blood on ICE until centrifugation.

Patient Prep

Within 30 (±5) minutes of venipuncture, centrifuge the collection tube at 1600 (± 200) x g (rcf) at 4°C (± 2°C) for 15 (±1) minutes. Transfer the cell-free plasma to a clean cryogenic storage tube and immediately store the cryogenic tube at -20°C, or freeze at -60°C or below in an ultra-low freezer.

Preferred volume: 1 mL plasma
Minimum volume: 0.5 mL plasma

Pediatric Collection

Minimum volume: 0.25 mL plasma

Unacceptable Conditions

Thawed samples or samples stored at room temperature prior to shipment.

Storage Transport Temp

Ship samples overnight in a well-insulated container on dry ice.

Stability

-60°C or below in an ultra-low freezer - up to 1 year

Notes

This panel includes the following tests:

Interleukin 2 (aka IL2)
Interleukin 4 (aka IL4)
Interleukin 5 (aka IL5)
Interleukin 6 (aka IL6)
Interleukin 10 (aka I10)
Interleukin 12 (aka I12S)
Interferon Gamma (aka INFG)
Tumor Necrosis Factor alpha (aka TNFA)

Additional testing may be needed for less than perfect samples on confirmatory testing/reflex testing.

To prevent unnecessary delays in testing, please send one aliquot per test request.

Performed

Monday - Friday

Methodology

Luminex bead based assay

Turnaround Time

Up to 4 weeks

Department

Complement Lab - Cytokines

Synonyms

Complement (TH1/TH2 Panel A), Cytokine (TH1/TH2 Panel A), Cytokines (TH1/TH2 Panel A), THPNLA

Study Offerings

Not offered

Related Tests

THPB, TH1, TH2

Reference Interval

Interleukin 2 (IL-2): 0.76 – 2.82 pg/mL
Interleukin 4 (IL-4): 0.00 – 2.18 pg/mL
Interleukin 5 (IL-5): 0.00 – 6.89 pg/mL
Interleukin 6 (IL-6): 0.00 – 7.14 pg/mL
Interleukin 10 (IL-10): 0.00 -32.96 pg/mL
Interleukin 12 (IL-12 p70): 0.00 – 11.05 pg/mL
Tumor Necrosis Factor alpha (TNFA): 1.27 – 46.46 pg/mL
Interferon gamma (INFG): 0.00 - 26.79 pg/mL

Interpretation

Elevated cytokine levels could be indicative of an immune response