Advanced Diagnostic Laboratories

Cytokine Panel A

Test Code

THPA

Description

Cytokines are low molecular-weight proteins with numerous biological activities that are critical for immune defense, and immune surveillance. In general, Th1-type cytokines produce pro-inflammatory immune responses for killing intracellular infections such as viruses and bacteria. In contrast, Th2-type cytokines promote antibody production, eosinophil activation, and anti-inflammatory responses. Dysregulated cytokine production can lead to tissue damage, immune dysfunction, and disease progression.

Collect

Plasma from a lavender top (EDTA) tube is the only acceptable sample type. Mix sample thoroughly. Keep tubes cold on wet ice or with a cold pack after collection.

Patient Prep

Within 30 minutes of venipuncture, centrifuge the collection tube at 1600 (±200) x g (rcf) at 4°C (± 2) for 15 (± 1) minutes. Transfer the cell-free plasma to a clean tube and immediately store samples on dry ice or at -60°C or below in an ultra-low freezer.

Preferred volume: 1 mL

Ambient temperature for centrifugation is acceptable provided that the time between collection and storage is minimized (approximately 30 minutes).

Pediatric Collection

Minimum volume: 0.5 mL plasma, frozen.

Unacceptable Conditions

Thawed specimen or specimen stored at room temperature prior to shipment.

Storage Transport Temp

Send frozen plasma Priority Overnight via FedEx and in a well insulated container on dry ice.

Stability

-60°C or below in an ultra-low freezer - up to 1 year

Notes

This panel includes the following tests:

Interleukin 2 (aka LIL2)
Interleukin 4 (aka LIL4)
Interleukin 5 (aka LIL5)
Interleukin 6 (aka LIL6)
Interleukin 10 (aka LIL10)
Interleukin 12 (aka LIL12S)
Interferon Gamma (aka LINFG)
Tumor Necrosis Factor alpha (aka LTNFA)

Additional time may be needed for confirmatiory or reflex testing.

Performed

Monday - Friday

Methodology

Luminex bead based assay

Turnaround Time

up to 4 weeks

Department

Complement Lab - Cytokines

Synonyms

Complement (TH1/TH2 Panel A), Cytokine (TH1/TH2 Panel A), Cytokines (TH1/TH2 Panel A), THPNLA

Study Offerings

CAP/CLIA

Reference Interval

Interleukin 2 (IL-2): 0.00 – 1.59 pg/mL
Interleukin 4 (IL-4): 0.00 – 2.18 pg/mL
Interleukin 5 (IL-5): 0.00 – 9.02 pg/mL
Interleukin 6 (IL-6): 0.00 – 7.14 pg/mL
Interleukin 10 (IL-10): 0.00 -14.39 pg/mL
Interleukin 12 (IL-12 p70): 0.00 – 11.05 pg/mL
Tumor Necrosis Factor alpha (TNFA): 1.27 – 46.46 pg/mL
Interferon gamma (INFG): 0.00 - 10.74 pg/mL

Interpretation

Elevated cytokine levels could be indicative of an immune response