Scleroderma (Systemic sclerosis) Panel 12
Test Code
SSP12
Description
Collect
Collect in a plain red top or SST tube
Specimen Preparation
Allow sample to clot at room temperature for 20 to 60 minutes. Centrifuge ASAP to separate serum from cells and aliquot cell-free serum into a labeled polypropylene or similar plastic tube.
Preferred volume: 0.5 mL serum
Pediatric Collection
Pediatric Collection
Minimum volume: 0.1 mL
Storage Transport Temp
Send serum Priority Overnight via FedEx and in a well-insulated container frozen on dry ice.
Stability
Frozen at -70°C: 1 year. Serum samples may be stored at +2°C to +8°C for up to 14 days if necessary.
Notes
National Jewish Health Advanced Diagnostic Laboratories offers a Scleroderma (systemic sclerosis) Panel to aid in the diagnosis of systemic sclerosis. When combined with clinical features, the Scleroderma Antigen Panel can aid clinicians in making a diagnosis of systemic sclerosis and related conditions. The Scleroderma Antigen Panel provides a qualitative in vitro assay for human antibodies present in serum of the IgG class to 12 different antigens.
Performed
Weekly
Methodology
Immunoblot
Reported
2-12 days
Department
Immunology Lab - DiagnosticSynonyms
Scl-70, CENP A, centromere A, CENP B, centromere B, RP11, RP155, Fibrillarin, NOR90, Th/To, PM/Scl-100, PM/Scl-75, Ku, Ro-52
Reference Interval
Negative 0-5 Units
Borderline 6-10 Units
Positive 11-50 Units
Strong Positive >50 Units
Interpretation
Antibodies to the antigens are determined by an immunoblot assay. The color developed for each antigen is determined by densitometry and the intensity of the color is proportional to the antibody reactivity. The results are given in arbitrary intensity units.
CPT Code
84182