Advanced Diagnostic Laboratories

SC5b-9 Level-Terminal Complement Complex (Urine)

Test Code

SC59U

Description

Complement mediated renal diseases such as C3 glomerulopathy (C3G), atypical hemolytic uermic syndrome (aHUS), lumpus nephritis (LN) and immunoglobulin A nephropathy (IgAN) are characterized by dysregulated complement activation and increased complement activiation products, including SC5B-9.

Complement activation via the classical, alternative, or lectin pathways culminates in the formation of a soluble C5b-9 protein complex (sC5b-9), also known as the Terminal Complement Complex (TCC) or the Membrane Attack Complex (MAC). This assay quantitates the amount of urine sC5b-9 in relation to urine creatinine, and can therefore assess the level of terminal complement activation across all three pathways.

Collect

Urine collected via clean-catch midstream technique at first void in a sterile urine cup; place on ice ASAP following collection
Transfer urine to clean centrifuge on ice

Pediatric Collection

Minimum 500 µL

Unacceptable Conditions

Thawed specimen

Storage Transport Temp

Send frozen urine Priority Overnight via FedEx in a well-insulated container on dry ice

Stability

Frozen at -60°C or lower: >6 months

Methodology

ELISA (Urine sC5b9)

Modified Rate Jaffe (Urine Creatinine)

Turnaround Time

Up to 3 weeks

Department

Complement Lab

Synonyms

Terminal Complement Complex (TCC); ELISA (SC5b-9 Level – terminal complement complex); Complement (SC5b-9 Level – terminal complement complex); Complement Urine; Soluble C5b-9 Complex; Membrane Attack Complex (MAC)