Myositis 17 Antigen Panel

Test Code

MYOS

Description

Collect

Collect in a plain red top or SST tube.

Specimen Preparation

Allow sample to clot at room temperature for 20 to 60 minutes. Centrifuge ASAP to separate serum from cells and aliquot cell-free serum into a labeled polypropylene or similar plastic tube.

Preferred volume: 0.5 mL serum

Pediatric Collection

Minimum volume: 0.1 mL

Storage Transport Temp

Send serum Priority Overnight via FedEx and in a well insulated container frozen on dry ice.

Stability

Frozen at -70°C: 1 year. Serum samples may be stored at +2°C to +8°C for up to 14 days if necessary.

Notes

National Jewish Health Advanced Diagnostic Laboratories offer a Myositis Antigen Panel to aid in the diagnosis of the idiopathic inflammatory myopathies, commonly referred to as myositis. When combined with clinical features, the Myositis Antigen Panel can aid clinicians in making a diagnosis of myositis, polymyositis, dermatomyositis, anti-synthetase syndrome and related conditions. The Myositis Antigen Panel provides a qualitative in vitro assay for human antibodies present in serum of the IgG class to 17 different antigens.

Performed

Weekly

Methodology

Immunoblot

Reported

2-12 days

Department

Immunology Lab - Diagnostic

Synonyms

Mi-2 alpha; Mi-2 beta; Ku; PM-Scl100; PM-Scl75; Jo-1; SRP; PL-7; PL-12; EJ; OJ; Ro-52; TIF1 gamma; MDA5; NXP2; SAE1; cN-1A

Reference Interval

Negative 0-5 Units
Borderline 6-10 Units
Positive 11-50 Units
Strong Positive >50 Units

Interpretation

Antibodies to the antigens are determined by an immunoblot assay. The color developed for each antigen is determined by densitometry and the intensity of the color is proportional to the antibody reactivity. The results are given in arbitrary intensity units.