Lymphocyte Proliferation to Nickel
Test Code
NILPT
Description
Nickel lymphocyte proliferation testing is a clinically useful tool for evaluating patients undergoing initial joint replacement surgery or revision.
Specifically, patients with a prior history of sensitivity to metals can be tested before the initial joint replacement to help choose the right hardware. Patients also can be tested after joint replacement if they experience persistent pain and swelling, once an infection or a mechanical failure has been excluded, to determine if hypersensitivity to nickel in the hardware is the cause.
Collect
Preferred volume: 30 mL blood. Collect blood in three 10 mL Green (Sodium Heparin) top tubes. Gently invert several times to mix and prevent clots.
Patient Prep
POSITIVE PATIENT IDENTIFICATION [Labeling] requires we use two (2) pieces of information [identifiers].
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The standard two patient identifiers for ADx Laboratories are patient FULL NAME and DATE OF BIRTH.
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Limited exception for de-identified samples would be the sample NUMBER and patient DATE OF BIRTH.
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Both identifiers must match exactly on the specimen label and the requisition/order that corresponds to that specimen.
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Where appropriate for multiple samples submitted together, specimen collection information (e.g. site or source, date and time of collection, etc.) must also be included on the specimen label. Shipping kits may be obtained by calling ADx Client Services. Samples accepted Monday - Friday.
Pediatric Collection
Minimum volume: 20 mL blood. Two 10 mL Green (Sodium Heparin) top tube.
Unacceptable Conditions
Centrifuged specimens, specimens received after 24 hours of draw, frozen specimens, specimens in lithium heparin, specimens in tubes with gel such as SST's and PSTs.
Storage Transport Temp
Ship at ambient temperature Priority Overnight via FedEx and in a well insulated container.
Specimen must be received in the laboratory within 24 hours of collection.
Stability
Ambient: 24 hours
Notes
For more than 15 patient samples, ADx Client Services must be called at 800.550.6227 to schedule testing before sample is shipped. Alternatively, you may send a fax to 800.652.9556. Please include the number of specimens you anticipate sending. We accept samples Monday through Saturday.
Performed
Monday - Sunday
Methodology
Lymphocyte stimulation in cell culture
Turnaround Time
14 days
Department
Beryllium Lab
Synonyms
Lymphocyte Proliferation to Nickel, Blood, Lymphocyte Transformation to Nickel, Blood, Nickel LPT (Lymphocyte Stimulation to Nickel, Blood), NICKLT
Study Offerings
RUO
CAP/CLIA
Reference Interval
Non-Reactive
CPT Code
86353 x 9
New York Approved
No: Run with Waiver
FDA
LDT