Lymphocyte Proliferation to PHA, ConA, PWM
Test Code
LMITO
Shipments greater than 15 need to be scheduled with lab prior to sending.
Description
This assay assesses the ability of T and B lymphocytes to respond to mitogenic stimulation (PHA, ConA and PWM). PHA and ConA preferentially stimulate T cells and PWM preferentially stimulates B cells. The test is used to evaluate patient immune status and responsiveness.Collect
Draw whole blood into Sodium Heparin (green top) tube.Patient Prep
Preferred volume: 10 mLPediatric Collection
Minimum volume: 2-3 mL bloodUnacceptable Conditions
Centrifuged specimens, specimens received after 30 hours of draw, frozen specimens, specimens in lithium heparin, specimens in tubes with gel such as SSTs and PSTs.
Storage Transport Temp
Maintain and transport specimens at room temperature (18-22°C).
Ship specimens Priority Overnight via Fed Ex in container sufficiently insulated to avoid temperature extremes (<10°C and >37°C).
Stability
30 hours at room temperature (18-22°C)
Notes
This panel tests three mitogens: Phytohemagglutinin (PHA), Concanavalin A (ConA) and Pokeweed mitogen (PWM).Performed
Tuesday - Saturday
Methodology
Lymphocyte stimulation in cell culture using uptake of a radiolabeled nucleotide (tritiated thymidine)Turnaround Time
14 days
Department
Immunology Lab - Functional Assay
Synonyms
Lymphocyte stimulation (Lymphocyte Proliferation)Study Offerings
RUO
CAP/CLIA
Related Tests
LPHA, LCONA, LPWM, LMITO, LCAND, LTET, LANTI, WBPHA, WBCON, WBPWM, WBMIT, WBCAN, WBTET, WBANTReference Interval
By Report
Interpretation
Responses are compared with the laboratory-established normal range. A response is considered to be normal if 2 out of 3 conditions are within the normal range. An uninterpretable result may be due to an elevated response to medium alone.CPT Code
86353 x3
New York Approved
No: Run with Waiver
FDA
LDT