Lymphocyte Proliferation to Chromium - Blood
Test Code
CRLPT
Description
Chromium lymphocyte proliferation testing is a clinically relevant tool for the diagnosis of allergic contact dermatitis or chromium sensitivity. It can be used in situations where patch testing is not recommended. Prolonged exposure to chromium can result in occupational asthma, respiratory irritation, damage to several organs, or respiratory cancers.
Collect
Preferred volume: 30 mL blood. Collect blood in three 10 mL Green (Sodium Heparin) top tubes. Gently invert several times to mix and prevent clots.
If ordering multiple metals (Nickel LPT and/or Cobalt LPT): 30 mL blood for 1 metal, 20mL blood per each additional metal.
Patient Prep
POSITIVE PATIENT IDENTIFICATION [Labeling] requires we use two (2) pieces of information [identifiers].
- The standard two patient identifiers for ADx Laboratories are patient FULL NAME and DATE OF BIRTH.
- Limited exception for de-identified samples would be the same sample NUMBER and patient DATE OF BIRTH.
- Both identifiers must match exactly on the specimen label and the requisition/order that corresponds to the specimen
- Where appropriate for multiple samples submitted together, specimen collection information (e.g. site or source, date and time of collection, etc.) must also be included on the specimen label. Samples accepted Monday-Saturday.
Pediatric Collection
Preferred volume: 30 mL blood. Collect blood in three 10 mL Green (Sodium Heparin) top tubes. Gently invert several times to mix and prevent clots.
If ordering multiple metals (Nickel LPT and/or Cobalt LPT): 30 mL blood for 1 metal, 20 mL blood per each additional metal.
Unacceptable Conditions
Centrifuged specimens, specimens received after 24 hours of draw, frozen specimens, specimens in lithium heparin, specimens in tubes with gel such as SST's and PST's.
Storage Transport Temp
Ship at ambient temperature Priority Overnight via FedEx and in a well insulated container.
Specimen must be received in the laboratory within 24 hours of collection.
Stability
Ambient: 24 hours
Performed
Monday - Saturday
Methodology
Lymphocyte stimulation in cell culture
Turnaround Time
14 days
Department
Immunology Lab - Functional Assay
Synonyms
Lymphocyte Proliferation to Chromium, Blood, Lymphocyte Transformation to Chromium, Blood, Lymphocyte LPT (Lymphocyte Stimulation to Chromium, Blood)
Study Offerings
RUO
CAP/CLIA
Reference Interval
SI <2 Normal
SI ≥2 Abnormal
Interpretation
Abnormal: Abnormal lymphocyte proliferation to chromium sulfate.
Normal: Normal lymphocyte proliferation to chromium sulfate.
Borderline: Borderline lymphocyte proliferation to chromium sulfate. Repeat testing is recommended.
Uninterpretable: Uninterpretable response to chromium sulfate. Repeat testing is recommended.
CPT Code
86353 x 9
New York Approved
No: Run with Waiver
FDA
LDT