Interferon Gamma and IL-17 Producing CD4 T Cells by Flow Cytometry
Test Code
TH17C
Description
Collect
Draw one green top tube (Sodium or Lithium Heparin).
In addition, draw one green top tube from an unrelated donor to serve as a control.
Specimen Preparation
Do not centrifuge! Keep at room temp (18-22°C).
Preferred volume: 10 mL
Pediatric Collection
Minimum volume: 6 mL blood
Unacceptable Conditions
Frozen, clotted, hemolyzed or centrifuged specimens. Anticoagulants other than heparin. Specimens >24 hrs old.
Storage Transport Temp
Maintain and transport specimens at room temperature (18-22°C).
Ship specimens Priority Overnight via Fed Ex in container sufficiently insulated to avoid temperature extremes (<10°C and >37°C).
Stability
24 hours at room temperature (18-22°C)
Notes
Samples accepted Mon-Thur, no later than noon on Thursday. Samples must arrive within 24 hours of collection.
Performed
Mon-Thur
Methodology
Flow Cytometry
Reported
7-9 days
Department
Immunology Lab - Functional AssaySynonyms
Flow Cytometry (Interferon Gamma and IL-17 Producing CD4 T Cells);TH17 (Interferon Gamma and IL-17 Producing CD4 T Cells)
Reference Interval
- TH17 > 0.3% of CD4 T cells
- TH1 > 5.3% of CD4 T cells
Interpretation
Mutations of the gene encoding STAT3 are associated with the Hyper IgE syndrome (HIES). Because development of TH17 cells is dependent on STAT3, patients with HIES may have depressed levels of TH17 cells, thereby resulting in a compromised ability to fight infections. Analysis of the percentage of circulating TH17 cells assists in the evaluation of HIES.
CPT Code
86353, 88184, 88185