Advanced Diagnostic Laboratories

Extractable Nuclear Antigens

Test Code

ENA6

Description

Collect

Collect in a plain red top or SST tube.

Specimen Preparation

Separate serum from cells immediately by centrifugation and aliquot into a polypropylene or similar plastic tube.
Preferred volume: 1 mL serum

Pediatric Collection

Minimum Volume: 0.5mL serum

Unacceptable Conditions

Plasma, lipemic, grossly hemolyzed, microbially contaminated specimens, or specimens with visible particulates should not be used.

Storage Transport Temp

Frozen. Send serum Priority Overnight via FedEx and in a well insulated container on dry ice.

Stability

Up to 8 hours ambient or 48 hours refrigerated at 2-8°C. Freeze at or below -20°C for longer storage or shipping.

Notes

Positive ANAs will automatically reflex to ENA6 and DSDNA, but ENA6 (and DSDNA) may also be ordered independently.

The ENA6 screening profile includes IgG antibodies to Sm/RNP, Sm, SS-A, SS-B, Scl-70 and Jo-1, measured by ELISA.

All positive ENA6 tests will automatically reflex to individual components RNP, Sm, SS-A and SS-B, but not to Scl-70 and Jo-1.

All components except Jo-1 may also be ordered separately.

Performed

Monday through Friday

Methodology

ELISA

Reported

7 day turn around

Department

Immunology Lab - Diagnostic

Synonyms

ELISA (ANA Profile); IFA (ANA Profile)

Related Tests

ANA, RNP, Sm, SS-A, SS-B, Scl-70, MYOS (includes Jo-1), DSDNA