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Advanced Diagnostic Laboratories

Culture of Donor Bone Tissue

Test Code



This test is a culture for acid fast bacilli (AFB) in donor bone tissue. This test is used for identifying contamination of donor bone matrices with mycobacteria, especially M. tuberculosis.  

AFB culture is the inoculation of donor bone specimen onto multiple media types (including solid and liquid media) followed by monitoring for six weeks. Growth of any mycobacteria is identified for M. tuberculosis and other nontuberculous mycobacteria (NTM). These organisms are identified using first-line assays (i.e. no sequencing is performed for a higher-level identification). Note that a smear from the donor sample is not performed.