Advanced Diagnostic Laboratories

Anti-Neutrophil Cytoplasmic Antibody

Test Code

ANCA

Description

Granulomatosis with polyangiitis (GPA) is a rare, systemic, autoimmune vascular disease characterized by inflammation in small and medium sized blood vessels and by necrotizing granulomas primarily in the respiratory tract and kidneys. Antibodies that bind to cytoplasmic antigens in human neutrophils (ANCA) are useful serologic markers of GPA. ANCA have classically been divided into c-ANCA (cytoplasmic) and p-ANCA (perinuclear) patterns depending on the immunofluorescence patterns observed when patient serum is added to microscope slides coated with neutrophils.

Collect

Serum separator tube or a red top tube.

Patient Prep

Preferred volume: 1 mL serum

Pediatric Collection

Minimum Volume: 0.5mL serum

Unacceptable Conditions

Plasma, lipemic, grossly hemolyzed, microbially contaminated specimens, or specimens with visible particulates should not be used.

Storage Transport Temp

Frozen. Send serum Priority Overnight via FedEx and in a well insulated container on dry ice.

Stability

Up to 8 hours ambient or 48 hours refrigerated at 2-8°C. Freeze at minus 20°C or lower for longer storage or shipping.

Notes

This test includes screen for cytoplasmic and perinuclear antibodies.

Performed

Weekly

Methodology

Indirect Fluorescent Antibody (IFA), CYNT

Turnaround Time

7-9 days

Department

Immunology Lab - Diagnostic

Synonyms

IFA (Anti-Neutrophil Cytoplasmic Ab), Myeloperoxidase Ab (MPOAB), Proteinase 3 Ab (PR3)

Study Offerings

CAP/CLIA

Related Tests

ANA pattern and titer (ANA), C-reactive protein (CRP), Anti-cyclic citrullinated peptide antibodies (CCP), C4a level (C4AR, marker of acute Lyme disease), Rheumatoid factor antibody titer (RF), Erythrocyte sedimentation rate (ESR), Uric acid (UUAR), CD20, pan B cells {Rituxan monitor} (CD20), Anti-ds-DNA antibody (DSDNA), Sjogrens antibody, anti SSA, anti SSB (SSAB/SSBB), Anti-Scl 70 antibodies (SCL70), Myeloperoxidase Ab (MPO), Proteinase 3 Ab (PR3)