Advanced Diagnostic Laboratories

Collect

Plain red top or SST tube.

Specimen Preparation

Frozen specimens must be completely thawed before mixing. Mix thawed specimens by inverting 10X or low speed vortexing. If specimen contains fibrin, red blood cells, or particulate matter then centrifuge at ≥ 10,000 RCF (Relative Centrifugal Force) for 10 minutes.

Pediatric Collection

Minimum Volume: 0.4 mL serum

Unacceptable Conditions

Gross Hemolysis, Lipemia. High Triglycerides, heat-activated, pooled specimens

Storage Transport Temp

Offsite: Send frozen serum Priority Overnight via FedEx and in a well insulated container on dry ice.

Stability

Stability after cell separation:
3 days at RT (15-25°C)
1 week at 2-8°C
1 month frozen at -20°C

Performed

Monday & Thursday

Methodology

The Abbott Architect AFP assay is a chemiluminescent microparticle immunoassay (CMIA) for the quantitative determination of alpha fetoprotein (AFP). Patient results determined by assays using different manufacturer or methods may not be compareable.

Reported

4 hour routine, 1 hour STAT

Department

Core Lab

Synonyms

AFP

CPT Code

82105