6-Hour Prednisolone Kinetics
Test Code
PKPDK
Description
Therapeutic Drug Monitoring (TDM) of prednisolone is performed to optimize dosing, minimize toxicity, and ensure therapeutic efficacy in patients receiving this corticosteroid. Prednisolone is commonly used for inflammatory and autoimmune conditions.
Collect
Collect Serum in an 8-10 mL plain red top tube. Any other type of tube may influence accurate drug level quantification.
Patient Prep
Separate serum from cells immediately by centrifugation and aliquot into a polypropylene or similar plastic tube. Use a separate tube for each test ordered. Allow room for expansion of sample inside tube.
Preferred Volume: 2.0 mL of serum
Minimum Volume: 1.0 mL of serum
Pediatric Collection
If minimum volume cannot be obtained, samples can be ran with dilution calculations. 5x maximum dilution to be allowed.
Unacceptable Conditions
Thawed samples for greater than 24 hours
Storage Transport Temp
Fully complete PK requisition, Missing elements may delay resulting.
Ship samples via overnight delivery with appropriate amount of dry ice to remain frozen upon arrival.
Stability
Freeze at -20C. Up to 24 hours at room temperature.
Notes
Ship samples to be received Monday through Friday. Do not ship on Friday or Saturday.
Performed
Monday - Friday
Methodology
HPLC-UV
Turnaround Time
Within 10 business days. Weekends and holidays may delay results until following business day.
Department
Pharmacokinetics Lab
Synonyms
HPLC (Prednisolone Kinetics), pharmacokinetics (Prednisolone Kinetics), cortisol
Study Offerings
RUO
CAP/CLIA
Reference Interval
By Report
CPT Code
80299
New York Approved
No: Run with Waiver
FDA
LDT