Collect in a plain red top or SST tube.
Allow sample to clot at room temperature for 20 to 60 minutes. Centrifuge ASAP to separate serum from cells and aliquot cell-free serum into a labeled polypropylene or similar plastic tube.
Preferred volume: 0.5 mL serum
Minimum volume: 0.1 mL
Send serum Priority Overnight via FedEx and in a well insulated container frozen on dry ice.
Frozen at -70°C: 1 year. Serum samples may be stored at +2°C to +8°C for up to 14 days if necessary.
National Jewish Health Advanced Diagnostic Laboratories offer a Myositis Antigen Panel to aid in the diagnosis of the idiopathic inflammatory myopathies, commonly referred to as myositis. When combined with clinical features, the Myositis Antigen Panel can aid clinicians in making a diagnosis of myositis, polymyositis, dermatomyositis, anti-synthetase syndrome and related conditions. The Myositis Antigen Panel provides a qualitative in vitro assay for human antibodies present in serum of the IgG class to 16 different antigens.
Immunology Lab - Diagnostic
Mi-2 alpha; Mi-2 beta; Ku; PM-Scl100; PM-Scl75; Jo-1; SRP; PL-7; PL-12; EJ; OJ; Ro-52; TIF1 gamma; MDA5; NXP2; SAE1; cN-1A
Negative 0-5 Units
Borderline 6-10 Units
Positive 11-50 Units
Strong Positive >50 Units
Antibodies to the antigens are determined by an immunoblot assay. The color developed for each antigen is determined by densitometry and the intensity of the color is proportional to the antibody reactivity. The results are given in arbitrary intensity units.
84182 x 16