Lab resources and services
Dr. Redente’s laboratory is located in Smith building on National Jewish Health’s campus and offers several unique research methods and animal models for pulmonary basic science and translational research.
Our laboratory has expertise in the creation of novel inducible transgenic mouse lines that target fibroblast subpopulations both for advanced lineage tracing, for the deletion genes and overexpression of genes. These tools provide our research group with advanced methods to use for the analysis of our proposed questions. My laboratory is particularly interested in the diversity of pulmonary fibroblast sub-populations in the lungs and how these are changing during fibrosis and resolution. We have developed novel flow cytometry strategies to identify fibroblast subpopulations to study them throughout the time course of disease.
- In vivo fibrosis models (bleomycin, silica, BHT)
- In vivo models of targeted cell death via the human diphtheria toxin receptor
- In vitro primary cell culture and organoids
- Precision cut lung slices (PCLS) from murine and human
- Microscopy: Confocal, Atomic Force
- Flow Cytometry
- Genetic models of fibrosis and genetic lineage tracing
- Small animal microCT
- Matrix proteomics
- Transcriptomics (bulk, single cell and spatial sequencing)