Method to Produce Monoclonal Antibodies to Human T-Cell Receptor Elements Download HRPP SOPs Clinical Trials Find a Researcher Tech ID: 91-02 Summary The specificity of T-cell receptor to an antigen is determined by the five variable elements of the a and ß chains. Research scientists at National Jewish Health have developed a method to produce monoclonal antibodies against any of the five variable elements of human T-cell receptor. This method relies on the transfection of human/mouse hybrid T-cell receptor a or ß chains genes into a mouse T-cell hybridoma lacking an endogenous a or b chain gene. Potential Applications Research reagents. Monoclonal antibodies for therapeutic use. Advantages of Invention Simple method Method adaptable to any of the five variable elements of the human T-cell receptor. State of Development Scientists have demonstrated that using this method: hVß elements can be expressed on the surface of mouse T-cell hybridomas. The transfected T-cell hybrids are excellent immunogens in mice for hVß. Monoclonal antibodies against hVß13.1 and -13.2 can be raised and be used for the measurements of Vß13.1 and -13.2 in humans. Licensing Potential Available for licensing on a non-exclusive basis. Patent Status French Patent #0542897. Inventors Yongwon Choi, PhD, John Kappler, PhD and Philippa Marrack, PhD Publication Choi et al. Proc Natl Acad Sci U S A. 1991 Oct 1; 88(19):8357-61. PMID: 1656442 For Further Information, Contact: Emmanuel Hilaire, PhD Director Technology Transfer Office National Jewish Health 1400 Jackson Street, Room M206b Denver, CO 80206 Voice: (303) 398-1262 Fax: (303) 270-2352 HilaireE@njhealth.org