The Chu Laboratory is located on the 6th floor of the Smith building on the National Jewish Health campus and offers several unique research methods and animal models for pulmonary basic science research.

Research Methods

  • Generation of recombinant proteins
  • Gene promoter assay
  • Lentiviral gene knock-in and knock-down
  • Laser capture microdissection
  • Mouse models of live bacterial infection
  • Mouse tracheal epithelial cell isolation
  • Primary airway epithelial cell air-liquid interface cultures
  • Whole cigarette smoke system for cell cultures

Animal Models

  • Generated mouse strains (e.g., SPLUNC1 knockout mice)
  • Pathogens used in mouse models:
    • Human replication deficient adenovirus 5
    • Moraxella catarrhalis
    • Mycoplasma pneumoniae
    • Nontypeable Haemophilus influenzae (NTHi)
    • Pseudomonas aeruginosa