Bridges Laboratory Search Clinical Trials Find a Researcher Order a Test Research efforts in the Bridges laboratory are focused toward: 1) understanding the molecular regulation of epithelial cell specification and maturation during lung development; and 2) determining the molecular mechanisms that control pulmonary surfactant pool sizes in the postnatal and adult lung, with particular interest in rare pediatric and adult lung diseases associated with surfactant dysfunction. Our lab utilizes state-of-the-art methodologies including CRISPR/Cas9 gene editing in mice, AAV-mediated gene delivery, RNAseq, ATACseq and high content confocal microscopy coupled with physiological and biochemical analyses to address questions of direct clinical relevance. Lab Resources and Services Lab location K1004 Current Projects Role of GPR116 in Alveolar Homeostasis Editing Alveolar Progenitor Cells for Correction of Monogenic Disease Mechanisms of vGPCR mediated Cytomegalovius growth in the salivary gland , + × , James P. Bridges, PhD, is a researcher at National Jewish Health. Dr. Bridges is in the Division of Pulmonary, Critical Care and Sleep Medicine. View Full Profile Personnel Principal Investigator James P. Bridges, PhD Collaborators Jeffrey Whitsett, MD Chief, Section of Neonatology, Perinatal and Pulmonary Biology Cincinnati Children’s Hospital Medical Center Cincinnati, OH Alveolar epithelial cell repair following deletion of ABCA3 Sarah Wootton, PhD Associate Professor of Medicine University of Guelph, Ontario Veterinary College Guelph, ON, Canada Molecular virologist, targeting AAV vectors to alveolar epithelial cells Bill Miller, PhD Professor of Medicine University of Cincinnati College of Medicine Cincinnati, OH Viral GPCR signaling in salivary epithelial cells Publications Epithelial Gpr116 regulates pulmonary alveolar homeostasis via Gq/11 signaling. Brown K, Filuta A, Ludwig MG, Seuwen K, Jaros J, Vidal S, Arora K, Naren AP, Kandasamy K, Parthasarathi K, Offermanns S, Mason RJ, Miller WE, Whitsett JA, Bridges JP. JCI Insight. 2017 Jun 2;2(11). pii: 93700. doi: 10.1172/jci.insight.93700. eCollection 2017 Jun 2. Abstract A Novel Triple-Mutant AAV6 Capsid Induces Rapid and Potent Transgene Expression in the Muscle and Respiratory Tract of Mice. van Lieshout LP, Domm JM, Rindler TN, Frost KL, Sorensen DL, Medina SJ, Booth SA, Bridges JP, Wootton SK. Mol Ther Methods Clin Dev. 2018 Apr 14;9:323-329. doi: 10.1016/j.omtm.2018.04.005. eCollection 2018 Jun 15. Abstract Alveolar injury and regeneration following deletion of ABCA3. Rindler TN, Stockman CA, Filuta AL, Brown KM, Snowball JM, Zhou W, Veldhuizen R, Zink EM, Dautel SE, Clair G, Ansong C, Xu Y, Bridges JP, Whitsett JA. JCI Insight. 2017 Dec 21;2(24). pii: 97381. doi: 10.1172/jci.insight.97381. Abstract View all Publications Opportunities Research assistant and post-doctoral positions available, please inquire.