My lab studies transcriptional and epigenetic regulation of genes that control immune effector cell development and regulate cytokine and chemokine gene expression. We investigate how super-enhancers and their associated transcription factors integrate signals triggered by various external stimuli, such as antigenic stimulation, infection, cytokine and metabolic products, to generate gene transcription outputs. We also determine the contribution of genetic variants to enhancer activity, gene expression and disease severity. We utilize various cutting-edge technologies, including next-generation sequencing, bioinformatics, enhancer library and CRISPR to address significant research problems in inflammation and allergic immune responses.

 

Current Projects

Personnel

Principal Investigator
Hua Huang, MD, PhD

Research Fellow
Yapeng Li, PhD

Predoctoral Trainee
Jinyi Liang, BS

Undergraduate Students
Fathia Mohamed
Cydney Tinsley


View Former Lab Members

Open Positions

Our lab is currently recruiting graduate students, MD., Ph.D. students and postdoctoral fellows.

 

Contact Information

Dr. Hua Huang, MD, PhD
Department of Immunology and Genomic Medicine
National Jewish Health

1400 Jackson Street, K511
Denver, CO 80206

Office: 303.398.1281
Lab: 303.398.1287
huangh@njhealth.org

Publications

  • Li, Y., J. F. Gao, M. Kamran, L. Harmacek, T. Danhorn, S. M. Leach, B. P. O’Connor, J. R. Hagman, and Huang H. GATA2 regulates mast cell identity and responsiveness to antigenic stimulation by promoting chromatin remodeling at super-enhancers. Nature Communications in revision. Preprint, Bioaxiv, 10.1101/2020.09.16.300327, 2020.

  • Li, Y., Liu, B., Harmacek, L., Long, Z., Liang, J., Lukin, K., Leach, S., O'Connor, B., Gerber, A. N., Hagman, J., Roers, A. Fred D. Finkelman, F.D., and Huang, H. The transcription factors GATA2 and MITF regulate IgE/mast cell-mediated anaphylaxis by regulating Hdc gene expression in mast cells Hdc gene expression in mast cells and are required for IgE/mast cell-mediated anaphylaxis. J Allergy Clin Immunol, published online Dec 22, 2017, 10.1016/j.jaci.2017.10.043.

  • Li Y, Qi X, Liu B, and Huang H.  The STAT5-GATA2 Pathway Is Critical in Basophil and Mast Cell Differentiation and Maintenance. J Immunol. 2015, 194: 4328-38. (Also see Editorial in “In This Issue”)

  • Qi X, L. Chaves, Y.H. Zhuang, Y. Chen, D. Wang, J. Chahon, B. Graham, K. Ohmori, and Huang H.  Antagonistic regulation by transcription factors C/EBPa and MITF specifies basophil and mast cell fates.  Immunity, 2013, 39, 97-110.

  • Ohmori K, Y. Luo, Y. Jia, J. Nishida, Z. Wang, K. D. Bunting, D. Wang, and Huang H.  IL-3 Induces Basophil Expansion In Vivo by Directing Granulocyte-Monocyte Progenitors to Differentiate into Basophil Lineage-Restricted Progenitors in the Bone Marrow and by Increasing the Number of Basophil/Mast Cell Progenitors in the Spleen.  J Immunol 2009, 182: 2835-2841.

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