Loss of Allele, a quantitative PCR technique, will be used to rapidly identify putative positives for secondary screening.
This service includes the design, purchase, and validation of suitably positioned primer/probe pairs for your targeting construct, preparation of genomic DNA from targeted ES cell clones, and loss of allele (LOA) analysis. If the primer/probe set passes our validation tests (for primer efficiency), the screening of ES clones can be quite rapid, within a month or less.