Collect stool in sterile cup.
Preferred: 1 mL in Carey Blair medium transport container.
If possible avoid recent antibiotics, x-ray contrast material, antidiarrheal medications, antacids, bismuth or oil products. One stool per day for three days is recommended.
Refrigeration of stool specimens is not recommended since some bacteria, particularly Shigella, will not survive the acidic PH changes that occur with a drop in temperature.
If delay is more than 1 hour then transport in Cary-Blair media.
2 hours room temperature in sterile cup
Up to 7 days in Cary-Blair media
Refrigeration can retard bacterial growth, transport at RT.
7 days a week