Plasma from a lavender top (EDTA) tube is the only acceptable sample type. Mix sample thoroughly.
Centrifuge at room temp within one half hour of collection; preferably immediately after venipuncture. Transfer the cell-free plasma to a clean tube and immediately freeze the cell-free plasma on dry ice or at -70°C.
Preferred volume: 1 mL plasma, frozen
Minimum volume: 250 µL plasma, frozen
Send frozen plasma Priority Overnight via FedEx and in a well insulated container on dry ice.
Frozen at -70°C: 1 year
C4 MiniNeph: Nephelometry
up to 4 weeks
Nephelometry (Ratio of C4d to C4);Complement (Ratio of C4d to C4);ELISA (Ratio of C4d to C4);C4RAT
C4: 0.112-0.441 mg/mL
C4d: 0.52-7.88 mcg/mL
86160 X 2