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Home > Nucleic Acid Amplification Test (NAAT) (clinical specimen only)
Indicate specimen source: BAL, CSF, Sputum, Sputum (induced), Tissue (specify), Processed Specimen (specify) or Other Body Fluid (specify).
Indicate if patient is a Cystic Fibrosis patient (will require different decontamination), or if sample is environmental (contact laboratory before collection), or veterinary (animal type).
Sputum: >5 mL of unprocessed (raw) sputum. Collect the first early morning expectoration in a sterile 50 mL polypropylene centrifuge tube capable of withstanding 3,000 x g (i.e, Falcon, Greiner, etc).
Processed Sputum: As much processed sputum as possible (450µL needed for test). This is an acceptable specimen type for MTB2.
Body Fluids or CSF: As much body fluid as possible is aseptically collected by aspiration or during a surgical procedure. CSF should be maximum volume obtained, ideally at least 2 mL.
Gastric Aspirates: Must be neutralized (pH 7) with sodium carbonate if transport time exceeds four hours from collection. Transfer 5-10 mL to a sterile container.
Tissue: =10 g or as much as possible with a biopsy. Transfer to a sterile container (without formalin or preservatives).
Ship the specimen on the day of collection via airmail or by overnight courier.
Urine, Stool, and Blood are not tested with NAAT.
Patients who are suspected of having pulmonary TB based on clinical evaluation and who have received no antituberculosis therapy, <7 days of such therapy, or have not received such therapy in the last 12 months may be evaluated with NAAT.
Sputum: Three sputum specimens at 8-24 hour intervals and at least one first-morning specimen.Have patient rinse mouth with water before collecting sputum to minimize contaminating specimen with food particles, mouthwash, or oral drugs, which may inhibit the growth of mycobacteria.
Induced sputum: Use sterile hypertonic saline. Avoid sputum contamination with nebulizer reservoir water which may contain saprophytic mycobacteria from tap water. Specify Sputum (Induced) on requisition form, since it may resemble saliva which is not an acceptable specimen.
Bronchoalveolar lavage (BAL): Avoid contaminating bronchoscope with tap water.
CSF: Use maximum volume attainable, ideally at least 2 mL.
Gastric lavage: Aspiration of swallowed sputum from the stomach by gastric lavage may be necessary for infants, young children, and the obtunded. Fasting, early-morning specimens are recommended in order to obtain sputum swallowed during sleep. Samples of 5 to 10 mLl, adjusted to neutral pH, should be collected on 3 consecutive days.
Swabs are not recommended for the isolation of mycobacteria, since they provide limited material. They are acceptable only if a specimen cannot be collected by other means. Negative results obtained from swab specimens are unreliable.
Gastric aspirates that have not been neutralized are not acceptable.
Frozen sputum is not acceptable.
Urine, Stool and Blood are not tested with NAAT.
NAAT cannot performed on grossly bloody specimen.
Specimen that leaked in transit is not acceptable.
If specimen cannot be mailed on the day of collection, keep the specimen refrigerated until the day of shipment.
Specimen can be shipped at room temperature or cold,using a cool-pack.
Molecular detection by nucleic acid amplification
NAAT reported within 24 hours upon receipt or called-in request order.
Mycobacteriology Lab (TB)
AFB1, AFB3, AFB4, AFB5, MTB1, MTB2, MTB3, MTB4, MTB5, MTB6, NTM1, NTM2, NTM3, NTM4, NTM5
Nucleic Acid Amplification Test (NAAT) performed on first specimen or by request for subsequent specimens. Result: (MTB complex rRNA detected or No MTB complex rRNA detected).
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Advanced Diagnostic Laboratories
1400 Jackson St
Denver, CO 80206
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