Draw one green top tube (Sodium or Lithium Heparin).
In addition, draw one green top tube from an unrelated donor to serve as a control.
Do not centrifuge! Keep at room temp (18-22°C).
Preferred volume: 10 mL
Minimum volume: 6 mL blood
Frozen, clotted, hemolyzed or centrifuged specimens. Anticoagulants other than heparin. Specimens >24 hrs old.
Maintain and transport specimens at room temperature (18-22°C).
Ship specimens Priority Overnight via Fed Ex in container sufficiently insulated to avoid temperature extremes (<10°C and >37°C).
24 hours at room temperature (18-22°C)
Samples accepted Mon-Thur, no later than noon on Thursday. Samples must arrive within 24 hours of collection.
Immunology Lab - Functional Assay
Flow Cytometry (Interferon Gamma and IL-17 Producing CD4 T Cells);TH17 (Interferon Gamma and IL-17 Producing CD4 T Cells)
Mutations of the gene encoding STAT3 are associated with the Hyper IgE syndrome (HIES). Because development of TH17 cells is dependent on STAT3, patients with HIES may have depressed levels of TH17 cells, thereby resulting in a compromised ability to fight infections. Analysis of the percentage of circulating TH17 cells assists in the evaluation of HIES.
86353, 88184, 88185