Plain red top or SST tube.
Onsite [NJH]: Transport to lab within 1 hour.
Offsite: Separate serum from cells within 2 hours by centrifuge and aliquot into a labeled polypropylene or similar plastic tube.
Preferred volume: 1 mL serum
Minimum volume: 100 mcL serum
Offsite: Send frozen serum Priority Overnight via FedEx and in a well insulated container on dry ice.
Stability after cell separation:
8 hours at room temperature
24 hours refrigerated at 2-8°C
>24 hours frozen at -20°C
Nephelometry (Immunoglobulin G, total)
Primary Immunodeficiency Screen, Pneumococcal polysaccharide IgG antibody [12 serotypes] (PNU12), Diphtheria IgG antibody titer (DIPHT), Tetanus IgG antibody titer (TET), Lymphocyte enumeration, CDC panel [CD3/CD4, CD3/CD8, CD19, CD16/56] (TBCDC), CBC with automated diff (HCBC), Immunoglobulin A level (IGA), Immunoglobulin M level (IGM), Alternative complement pathway activity (AH50), Total complement activity (CH50)
Immunoglobulin G, Total
Newborn - 2 months
3 months - 1 year
2 years - 3 years
4 years - 19 years