Draw one green top (sodium or lithium heparin) or lavender top (EDTA) vacutainer tube.
Yellow top (ACD soln A or B )tubes are also acceptable but tube must be appropriately filled (because of liquid anticoagulant) to preserve sample integrity.
Handling: Do not centrifuge! Keep at room temp (18-22°C).
Preferred volume: 4mL
Minimum volume: 1mL blood
Frozen, clotted, hemolyzed, spun down, citrate anticoagulant (blue top)
Maintain and transport specimens at room temperature (18-22°C).
Ship specimens Priority Overnight via Fed Ex in container sufficiently insulated to avoid temperature extremes (<10°C and >37°C).
48 hours at room temperature (18-22°C).
CBC with Differential results from within 3 days of draw must also be provided or a lavender top included with required specimen to run CBC at ADx.
Samples are accepted Monday through noon on Fridays.
Immunology Lab - Phenotyping Assays
B Cell Enumeration;CD20 B Cells;Flow Cytometry (CD20);B Cell Marker
TBCDC, TBSBS, CD3SB, CD4M, CD48, NKMK, MEMB, XBCP
Lymphocyte Subset Normal Range Chart
This test is useful for assessing B Cell percentages and numbers and assists in the evaluation of humoral immunodeficiences. It is also useful for monitoring depletion of CD20 B cells following anti-CD20 monoclonal (e.g. Rituximab) therapy.