Draw one green top tube (Sodium or Lithium Heparin) top tube.
Do NOT centrifuge! Keep at room temperature (18-22°C).
Preferred volume: 4 mL
Minimum volume: 1 mL blood
Frozen, clotted, hemolyzed or centrifuged specimens. Anticoagulants other than heparin. Specimens >24 hrs old.
Maintain and transport specimens at room temperature (18-22°C).
Ship specimens Priority Overnight via Fed Ex in container sufficiently insulated to avoid temperature extremes (<10°C and >37°C).
24 hours at room temperature (18-22°C)
Samples must arrive within 24 hours of draw and are accepted Monday through noon on Friday.
Immunology Lab - Phenotyping Assays
chain (Mo1, Mac1, CR3) neutrophils (CD11b);Leukocyte Adhesion Deficiency (LAD);Neutrophil Adherence marker;iC3b-receptor (CD11b)
DHR, NBT, CTX, BACT, CD18, ADHM, CDA11, C11CO
CD11b: >154 Mean Fluorescene Intensity (MFI)
LAD-1 is an autosomal recessive disorder caused by mutations in CD18, the common chain of the beta-2 integrin family. The beta-2 integrin family is comprised of LFA-1 (CD11a/CD18), Mac-1/CR3 (CD11b/CD18) and p150/95 (CD11c/CD18). Defects in CD18 therefore affect the expression of CD11a, b and c. Reduction or loss of surface expression of the beta-2 integrins supports the diagnosis of LAD-1.