Serum from a plain red top is the only acceptable sample type.
Gel barrier tubes are not acceptable.
Allow blood to clot at room temp or 37°C for 20 to 60 minutes. Centrifuge to thoroughly remove cells and immediately transfer cell-free serum to a fresh tube and freeze the cell-free serum on dry ice or at -70°C.
Preferred volume: 1 mL
Minimum volume: 250 µL serum, frozen
Send frozen serum Priority Overnight via FedEx and in a well insulated container on dry ice.
Frozen at -70°C: 1 year
This is not a direct measurement of antibody quality, but only a qualitative (indirect) measure of its activity in causing C3 conversion.
Complement (C3 Nephritic Factor);2-D Immunoelectrophoresis (C3 Nephritic Factor)
Normal – Ratio is 0 – 0.26
Equivocal – Ratio is 0.27 – 0.33
Positive – Ratio is > 0.3
No male or female