Collect lavage fluid in a sterile container. DO NOT centrifuge until cell count and differential have been performed. Please provide results of cell count and differential to ADx Laboratories.
After cell count has been performed, centrifuge the lavage specimen (approximately 1200 rpm x 10 minutes).
Remove supernatant. Re-suspend cells in complete medium. This consists of:
Final concentration of cells in media should be approximately 5-10 million cells per mL. Place in a plastic centrifuge tube and seal.
For each sample sent, please enclose a requisition with the name of the patient.
POSITIVE PATIENT IDENTIFICATION [Labeling] requires we use two (2) pieces of information [identifiers].
Specimens received more than 24 hours old, frozen specimens, specimens in lithium heparin, specimens in tubes with gel such as SST's and PSTs.
Send ambient fluid Priority Overnight via FedEx in a well insulated container (or in the ADx shipping kit). Specimen must be received in the laboratory within 24 hours of collection.
Shipping kits may be obtained by clicking here - cost is $5 per kit.
Ambient: 24 Hours
ADx Client Services must be called at 800.550.6227 to schedule testing before sample is shipped. Scheduling with laboratory in advance of collection is preferred.
Lymphocyte stimulation in cell culture
Immunology Lab - Functional Assay
Lavage;Lymphocyte Proliferation (Beryllium Lymphocyte Stimulation, Lavage)