Test Code: AMIKP
One red top serum vacutainer (no gel)
Pharmacokinetic factors influence the correct time of sample collection after the last drug dose. These factors include; dosage form, mode of administration, concomitant drug therapy, and biological variations affecting drug disposition.
To obtain a serum amikacin concentration that best represents the peak tissue level, draw the sample one hour after an intramuscular injection, 30 minutes after ending a 30-minute intravenous infusion, or immediately after a 60-minute intravenous infusion.
When adjusting dosage, measure peak and trough levels during the same dosing interval.
Allow to clot for 30 minutes in an upright position prior to centrifugation. Centrifuge within 60 minutes of collection.
Minimum volume: 0.2mL serum
Preferred volume: 0.5mL serum
Moderate/severe hemolysis, sample not properly identified; incorrect container; insufficient sample volume.
Send refrigerated (2-8°C)
Before centrifugation: 1 hour RT
After centrifugation: Refrigerated (2-8C), 2 weeks.
Within 24 hours
FPIA (Amikacin);pharmacokinetics (Amikacin);HIV (Amikacin)
Optimal peak concentration