Collect in a plain red top or SST tube.
Allow sample to clot at room temperature for 20 to 60 minutes. Centrifuge ASAP to separate serum from cells and aliquot cell-free serum into a labeled polypropylene or similar plastic tube.
Preferred volume: 1 mL serum
Minimum volume: 0.5 mL
Samples maintained at room temperature >24 hours, samples maintained refrigerated >7 days.
Send serum Priority Overnight via FedEx and in a well insulated container at ambient temperature, with an ice pack or frozen on dry ice.
2 days ambient (18-23°C), 1 week refrigerated (2-8°C), 1 year frozen (-20C). One freeze-thaw cycle will not negatively affect testing.
0.25 mL serum is required for a single allergen. Add 0.05 mL for each additional allergen. ie: 1 mL will yield 15 allergens.
Immunofluorescence (Allergen IgE Chicken Feathers);RAST (Allergen IgE Chicken Feathers)