Isolates of mycobacteria or partially acid fast organisms in solid media such as Middlebrook slant (7H10 or 7H11), Lowenstein-Jensen medium slant, or in broth (7H9, MGIT, MP BACT/Alert, VersaTrek).
Specimen volume: Lab must receive visible growth of isolate on solid media or an isolate in broth media (≥3 mL).
Transport or mail isolates at ambient temperature. For nontuberculous mycobacteria (NTM), follow IATA/ DOT category B and for M. tuberculosis complex isolates, follow IATA/DOT category A requirements for shipping biological materials.
Dependent on method used; see methodology section.
Identification results are reported within 3 days to 2 weeks depending on which method is used.
Infectious Disease Laboratory, Mycobacteriology
Line probe assays, Hain, sequencing, identification, reverse hybridization assay
AFB1, AFB3, VETCX, ENVCX
NY State: Waiver/single use permit required for identification of AFB.
Organisms growing in pure cultures are identified to the species or subspecies level whenever possible or appropriate.
Below are different test methods used for identification of various types of AFB and partially acid fast organisms. Only the relevant test methods will be performed and charged for the organism recovered or sent to the lab.