Submit a slant or at least 2.0 mL of a broth culture. Specify the type of media used.
Mixed cultures or non-viable organisms; there is an additional charge for purification/isolation of cultures containing a non-acid-fast contaminant, or more than one mycobacterial species.
This test code is for identification of a positive AFB culture. Indicate specimen source of organism and any other pertinent information such as the growth rate of the organism, growth temperature, DNA probe results, or preliminary identification. The laboratory should be notified when the presence of M. genavense, M. haemophilum and other fastidious mycobacteria are suspected, as these organisms will not grow on media routinely used for subcultures.
If colony morphology on a solid medium resembles Mycobacterium tuberculosis (MTB) complex, a line probe assay to detect M. tuberculosis complex will be performed. If positive, first-line drug antimicrobial susceptibility testing (AST; MTB2) will be performed on all initial isolates. The laboratory will provide at no charge a subculture to the respective state laboratory for genotyping upon request.
A line probe assay to rule out the presence of M. tuberculosis will be performed on ALL liquid cultures submitted without preliminary identification unless a clinical history indicating nontuberculous mycobacterial (NTM) infection is provided or indicated on the requisition that M. tuberculosis has been ruled out.
All other mycobacteria will be identified using line probe assay or DNA gene sequencing (i.e., rpoB gene and 16S rRNA gene sequencing).
There is an additional charge for purification/isolation of cultures containing a non-acid-fast contaminant, or more than one mycobacterial species.
Seven days a week.
Identification of AFB
Variable depending on methodology
Mycobacteriology Lab (TB)
2d;AFB Identification by Accuprobe;AFB Identificatio by Line Probe (Hain Test);AFB Identification by DNA Probe;AFB Identification by Line Probe (Hain Test)
AFB3, AFB5, NTM1, NTM2, NTM3, NTM4, NTM5, MTB1, MTB2, MTB3, MTB4, MTB5, MTB6
Identification to the species level is done using a combination of procedures determined by the National Jewish Health laboratory (Amplified MTD, line probe, 16S rDNA sequencing, or rpoB sequencing).
AST performed on nontuberculous mycobacterium (NTM) isolates by request only.
Per Official ATS/IDSA Statement: Diagnosis, Treatment, and Prevention of Nontuberculous Mycobacterial Diseases (2007),M. gordonae is frequently encountered in the environment and in clinical laboratories but is almost always considered nonpathogenic; therefore, AST for M. gordonae is performed by specific request only.
Identification of M. tuberculosis complex 87150
Species within M tuberculosis complex 87153x1
NTM identification 87153x1
Species within M. abscessus 87153x1