Type 2 immunity, characterized by production of type 2 cytokine interleukin (IL)-4, IL-5 and IL-13, is thought to evolve to fight against parasitic infection. However, this type of immunity also causes allergic inflammation, which is the underlying cause of allergic disease, such as food allergy and asthma. Type 2 effectors include CD4+ helper type 2 cells (Th2), CD8+ cytotoxic type 2 cells (TC2), type 2 innate lymphoid cells (ILC2), IgE-producing B cells and type 2 granulocytes that include type 2 cytokine-producing eosinophils, basophils and mast cells. Our lab investigates how type 2 effector cells are made and how type 2 cytokine genes are regulated and applies knowledge gained to understanding why some individuals are more susceptible to develop allergy and asthma and why some allergy and asthma suffers develop more severe form of disease while others do not. We have also been interested in studying gene regulation in other T helper subsets and in the hematopoietic system. Our lab uses cutting edge technologies and innovative approaches to address challenging research questions in the research fields.
Li Y, Qi X, Liu B, and Huang H. The STAT5-GATA2 Pathway Is Critical in Basophil and Mast Cell Differentiation and Maintenance. J Immunol. 2015, 194: 4328-38. (Also see Editorial in “In This Issue”)
Xiaopeng Qi, Lee Chaves, Yonghua Zhuang, Yuhong Chen, Demin Wang, Jacob Chahon, Brian Graham, Keitaro Ohmori, and Hua Huang. Antagonistic regulation by transcription factors C/EBPa and MITF specifies basophil and mast cell fates. Immunity, 2013, 39, 97-110.
Zhihong Chen, Shanze Wang, Nkiruka Erekosima, Jessie Hong, Xiaopeng Qi, Yapeng Li, Patricia Merkel, Vijaya Nagabhushanam, Eugene Choo, Rohit Katial, Rafeul Alam, Hong Wei Chu, Yonghua Zhuang, Meiling Jin, Chunxue Bai, and Hua Huang. IL-4 confers resistance to IL-27-mediated suppression on CD4+ T cells by impairing Signal Transducer Activator of Transcription 1 signaling. J Allergy Clin Immunol, 2013;132:912-21. (Also see Editor’s Choice of the same issue.)
Keitaro Ohmori, Yuchun Luo, Yi Jia, Jun Nishida, Zhengqi Wang, Kevin D. Bunting, Demin Wang, and Hua Huang. IL-3 Induces Basophil Expansion In Vivo by Directing Granulocyte-Monocyte Progenitors to Differentiate into Basophil Lineage-Restricted Progenitors in the Bone Marrow and by Increasing the Number of Basophil/Mast Cell Progenitors in the Spleen. J Immunol 2009, 182: 2835-2841.
Zhang Y, Apilado R, Coleman J, Ben-Sasson SZ, Tsang S, Hu-Li J, Paul WE and Huang H. Inteferon gamma stabilizes the T helper cell type 1 phenotype. J Exp Med 2001,194(2):165-172.