Recent Publications

  1. Gould NS, White CW, Day BJ. A role for mitochondrial oxidative stress in sulfur mustard analog 2-chloroethyl ethyl sulfide-induced lung cell injury and antioxidant protection. J Pharmacol Exp Ther 328(3): 732-739, 2009.
  2. Tewari-Singh N, Rana S, Gu M, Pal A, Orlicky DJ, White CW, Agarwal R. Inflammatory biomarkers of sulfur mustard analogue 2-chloroethyl ethyl sulfide-induced skin injury in SKH-1 hairless mice. Toxicol Sci 108(1): 194-206, 2009.
  3. Pal A, Tewari-Singh N, Gu M, Agarwal C, Huang J, Day BJ, White CW, Agarwal R. Sulfur mustard analog induces oxidative stress and activates signaling cascades in the skin of SKH-1 hairless mice. Free Radic Biol Med 47(11): 1640-1651, 2009.
  4. O'Neill HC, White CW, Veress LA, Hendry-Hofer TB, Loader JE, Min E, Huang J, Rancourt RC, Day BJ. Treatment with the catalytic metalloporphyrin AEOL 10150 reduces inflammation and oxidative stress due to inhalation of the sulfur mustard analog 2-chloroethyl ethyl sulfide. Free Radic Biol Med 48(9): 1188-1196, 2010.
  5. Tewari-Singh N, Gu M, Agarwal C, White CW, Agarwal R. Biological and molecular mechanisms of sulfur mustard analogue-induced toxicity in JB6 and HaCaT cells: possible role of ataxia telangiectasia-mutated/ ataxia telangiectasia-Rad3-related cell cycle checkpoint pathway. Chem Res Toxicol 23(6): 1034-1044, 2010.
  6. Veress LA, O’Neill HC, Hendry-Hofer TB, Loader JE, Rancourt RC, White CW.  Airway obstruction due to bronchial vascular injury after sulfur mustard analog inhalation.  Am J Respir Crit Care Med 2010 182:1352-61.
  7. White CW, Martin JG. Chlorine gas inhalation: Human clinical evidence of toxicity and experience in animal models.  Proc Amer Thoracic Soc 2010;7(4):257-263.
  8. Jain AK, Tewari-Singh N, Orlicky D, White CW.  2-Chloroethyl ethyl sulfide causes microvesication and inflammation-related histopathological changes in male hairless mouse skin.  Toxicology, 2011;282:129-38.
  9. Tewari-Singh N, Agarwal C, Huang J, Day BJ, White CW, Agarwal R.  Efficacy of glutathione in ameliorating sulfur mustard analog-induced toxicity in cultured skin epidermal cells and in SKH-1 mouse skin in vivo. J Pharmacol Exp Ther. 2011 Feb;336(2):450-9.
  10. Rancourt RC, Veress LA, Guo X, Jones TN, Hendry-Hofer TB, White CW. Airway Tissue Factor-Dependent Coagulation Activity in Response to Sulfur Mustard Analog 2-chloroethyl ethyl sulfide (CEES).  Am J Physiol Lung Cell Mol Physiol. 2011 Sep 30. [Epub ahead of print] PMID: 21964405
  11. Inturi S, Tewari-Singh N, Gu M, Shrotriya S, Gomez J, Agarwal C, White CW, Agarwal R. Mechanisms of sulfur mustard analog 2-chloroethyl ethyl sulfide-induced DNA damage in skin epidermal cells and fibroblasts.  Free Radic Biol Med. 2011 Aug 26
  12. Jain AK, Tewari-Singh N, Gu M, Inturi S, White CW, Agarwal R.  Sulfur mustard analog, 2-chloroethyl ethyl sulfide-induced skin injury involves DNA damage and induction of inflammatory mediators, in part via oxidative stress, in SKH-1 hairless mouse skin.  Toxicol Lett. 2011 Sep 10;205(3):293-301.
  13. O'Neill HC, Orlicky DJ, Hendry-Hofer TB, Loader JE, Day BJ, White CW.  Role of reactive oxygen and nitrogen species in olfactory epithelial injury by the sulfur mustard analogue 2-chloroethyl ethyl sulfide.  Am J Respir Cell Mol Biol. 2011 Aug;45(2):323-31. 
  14. McGovern T, Day BJ, White CW, Powell WS, Martin JG.  AEOL10150: a novel therapeutic for rescue treatment after toxic gas lung injury. Free Radic Biol Med. 2011 Mar 1;50(5):602-8
  15. Kachadourian, R, Pugazhenthi, S, Velmurugan, K, Backos, DS, Franklin, CR, McCord JM and Day, BJ. 2',5'-Dihydroxychalcone-induced glutathione is mediated by oxidative stress and kinase signaling pathways. Free Radic Biol Med 51:1146-1154, 2011.





Abstracts and Posters

CounterACT Meeting June, 2011

  1. Tissue factor-dependent coagulation activity in response to 2-Chloro ethyl ethyl sulfide.  RC Rancourt, Livia A. Veress, Tara Hendry-Hofer, Tara Jones, Xling Guo, CW White
  2. Optimizing AEOL10150 for rescue of sulfur mustard (CEES)-induced oxidative airway injury.  Veress LA, Hendry-Hofer T, Min E, Huang J, Loader J, Garlick R, Rioux J, Rancourt R, White CW, Day BJ.

CounterACT Meeting June, 2010

  1. CEES Inhalation results in nasal olfactory epithelial damage: the role of nitration and oxidative stress. Heidi C. O'Neill, Tara B. Hendry-Hofer, Joan E. Loader, David J. Orlicky, Brian J. Day, and Carl W. White.

CounterAct Meeting in April, 2007

  1. CEES (2-Chloroethyl Ethyl Sulfide) inhalation: Disruption and adaption in sulfur metabolism.  Raymond C. Rancourt1, Heidi C. O’Neill1, Glen S. McConville1, Joan Loader1, Justin Hinde1, Sally J. Stabler2, Carl W. White1, and Brian J. Day1    1National Jewish Health, Denver, Colorado;2University of Colorado Denver, Denver Colorado.
  2. Inhalation of 2-chloroethyl ethylsulfide (CEES; half-mustard) causes necrotizing bronchiolitis.  O’Neill HC, Loader JE, Hinde JL, Rancourt RC, McConville GS, White CW. National Jewish Health, Denver, CO 80206.
  3. Glutathione protection against toxicity of 2-chloroethyl ethylsulfide (CEES; half mustard) in human airway epithelial cells. Tara N. Jones#, Raymond C. Rancourt , Heidi C. O’Neill, Brian J. Day#, and Carl W. White # *    # National Jewish Health 1400 Jackson Street Denver, CO 80206    *Department of Pharmaceutical Sciences, Program in Toxicology University of Colorado Denver 4200 E 9th Avenue, C-238 Denver, CO 80262
  4. Characterization of a system for airway delivery of 2-chloroethyl ethylsulfide (CEES; half-mustard).   McConville G, Van Dyke M, Martyny J, Rancourt RC, O’Neill H, White CW, Day BJ, and R Jaeger.  National Jewish Health, Denver, CO 80206, and CH Technologies (CHT), Westwood, NJ 07675, USA.


The following were submitted for presentation at the Society of Toxicology (SOT) meeting for

(Submitted in 2011) for March 2012 SOT meeting:

  1. Mechanisms of sulfur mustard analogs, 2-chloroethyl ethyl sulfide and nitrogen mustard-induced DNA damage in skin cells.  Inturi S, Tewari-Singh N, Agarwal C, White CW*,  and Agarwal R.  University of Colorado Denver, Denver, CO; *National Jewish Health, Denver, CO.
  2. Histopathological and inflammatory changes in nitrogen mustard-induced mouse skin injury and possible role of myeloperoxidase in neutrophil-mediated inflammation.  Jain AK, Tewari-Singh N, Inturi S, Gu M, Orlicky DJ, White CW*, Agarwal A.  University of Colorado Denver, Denver, CO; *National Jewish Health, Denver, CO.
  3. Therapeutic efficacy of silibinin in attenuating sulfur mustard analog-induced skin injuries.  Tewari-Singh N, Jain AK, Inturi S, Agarwal C, White CW*, Agarwal R.  University of Colorado Denver, Denver, CO; *National Jewish Health, Denver, CO.


  1. Sulfur mustard analog-caused activation of signaling cascades in SKH-1 hairless mouse skin.  A. Pal, C. Agarwal, S. Rana, M. Gu, N. Tewari-Singh, C.W. White* and R. Agarwal.  University of Colorado Denver, Denver, CO; *National Jewish Health, Denver, CO.
  2. Biological and molecular markers for sulfur mustard analog CEES-induced skin injury in mouse and human epidermal keratinocytes.  N. Tewari-Singh, S. Rana, M. Gu, C. Agarwal, C.W. White* and R. Agarwal.  University of Colorado Denver, Denver, CO; *National Jewish Health, Denver, CO.
  3. Developing in vivo CEES-induced skin toxicity mouse models.  Rana S, Tewari-Singh N, Mallikarjuna GU, Orlicky DJ, *White CW, and Agarwal R.  University of Colorado Denver, Denver, CO; *National Jewish Health, Denver, CO

Other Significant Publications Related to Our Research

  1. Bifunctional Alkylating Agent-Induced p53 and Nonclassical Nuclear Factor kB Responses and Cell Death Are Altered by Caffeic Acid Phenethyl Ester: A Potential Role for Antioxidant/Electrophilic Response-Element Signaling.   Minsavage GD and Dillman III, JF.   The Journal of Pharmacology and Experimental Therapeutics 2007,321:202-212.
  2. Genomic Analysis of Rodent Pulmonary Tissue Following Bis-(2-chloroethyl) Sulfide Exposure. Dillman III, JF, Phillips CS, Dorsch LM, Croxton MD, Hege AI, Sylvester AJ, Moran TS, Sciuto AM.   Chem Res Toxicol. 2005,18:28-34.
  3. MALDI-ToF/MS as a Diagnostic Tool for the Confirmation of Sulfur Mustard Exposure.  Price EO, Smith, JR, Clark CR, Schlager JJ, Shih ML.   J Applied Toxicology.  2000,20:S193-S197.
  4. The Sources, Fate, and Toxicity of Chemical Warfare Agent Degradation Products.  Munro NB, Talmage SS, Griffin GD, Waters LC, Watson AP, King JF, Hauschild V.  Environmental Health Perspectives. 1999,107:933-974.
  5. Modulation of the Expression of Superoxide Dismutase Gene in Lung Injury by 2-Chloroethyl Ethyl Sulfide, a Mustard Analog.  Mukhopadhyay S, Rajaratnam V, Mukherjee S, Smith M, Das SK.   J Biochem Molecular Toxicology. 2006,20:142-149.
  6. Pretreatment of Human Epidermal Keratinocytes with D, L-Sulforaphane Protects Against Sulfur Mustard Cytotoxicity.  Gross CL, Nealley EW, Nipwoda MT, Smith WJ.  Cutaneous and Ocular Toxicology. 2006,25:155-163.
  7. Protective effect of various antioxidants on the toxicity of sulphur mustard administered to mice by inhalation or percutaneous routes.  Kumar O, Sugendran K, Vijayaraghavan R.    Chemico-Biological Interactions.  2001,134:1-12.
  8. Microarray Analysis of Mouse Ear Tissue Exposed to Bis-(2-chloroethyl) Sulfide: Gene Expression Profiles Correlate with Treatment Efficacy and An Established Clinical Endpoint.  Dillman III JF, Hege AI, Phillips CS, Orzolek LD, Sylvester AJ, Bossone C, Henemyre-Harris C, Kiser RC, Choi YW, Schlager JJ, Sabourin CL.   Journal of Pharmacology and Experimental Therapeutics.  2006,317:76-87.
  9. Modifications of breathing pattern induced by inhaled sulphur mustard in mice.  Vijayaraghavan R.    Arch Toxicol. 1997,71:157-164.
  10. Calmodulin mediates sulfur mustard toxicity in human keratinocytes.  Simbulan-Rosenthal CM, Ray R, Benton B, Soeda E, Daher A, Anderson D, Smith WJ, Rosenthal DS.    Toxicology. 2006,227:21-35.
  11. Wound Healing of Cutaneous Sulfur Mustard Injuries: Strategies for the Development of Improved Therapies.   Graham JS, Chilcott RP, Rice P, Milner SM, Hurst CG, Maliner BI.    Journal of Burns and Wounds.  2005,4:1-45.


For questions, contact us / Phone: 303.398.1617 / Fax: 303.270.2189