Research Tools:  Antibodies

Title and Inventors Description and publication
HIG-632.13

Kappler; Marrack

HIG-632.13 is a mouse IgG2b monoclonal antibody against Armenian hamster IgG1

(April 2008)

HIG-88.2

Kappler; Marrack

HIG-88.2 is a mouse IgG1 monoclonal antibody against Armenian hamster IgG2a and IgG2b

(April 2008)

HIG-65

Kappler; Marrack

HIG-65 is a mouse IgG1 monoclonal antibody against Armenian hamster IgG2b

(April 2008)

HIG-346

Kappler; Marrack

HIG-346 is a mouse IgG1 monoclonal antibody against Armenian hamster light chain lambda 1

(April 2008)

HIG-29

Kappler; Marrack

HIG-29 is a mouse IgG1 monoclonal antibody against Armenian hamster light chain kappa 1

(April 2008)

Mouse monoclonal antibody against human ECSOD (4G11G6)

Crapo; Bowler; Regan

4G11G6 was raised against human EC-SOD purified from aortas and can be used in ELISAs, Western Blot analyses, and immunohistochemistry.

Publication abstract 1; Publication abstract 2

(Feb 2008)

Monoclonal antibody against Ag/MHC receptor on D0-11.10 (KJI-26.1)

Kappler; Marrack

KJI-26.1 specifically recognizes the Ag/MHC receptor on D0-11.10, a chicken ovalbumin (cOVA)/I-Ad-reactive T cell hybridoma. Additional data provided upon request.

Publication abstract 1; Publication abstract 2

Hamster monoclonal antibody against all Mtv-7 superantigens (VS1-2.8)

Winslow; Kappler; Marrack

VS1-2.8 specifically recognizes the immediate amino terminus of potentially all mouse mammary tumor virus superantigens. Additional data provided upon request.

Publication abstract

Hamster monoclonal antibody against Mtv-7 superantigen (VS7)

Winslow; White; Kappler; Marrack

Publication abstract
Mouse monoclonal antibody against Lgp55 (PA3-795.4)

Golde; White; Kappler; Marrack

Lgp55 is a mouse cell surface glycoprotein which is involved in the interaction between T cells and some target cells. Additional data provided upon request.

Publication abstract

Monoclonal antibody (KJ12-98)

White; Kappler; Marrack

Publication abstract
Hamster IgG2 monoclonal antibody against p25 (MKBAC-165.2)

Crawford; White; Kappler; Marrack

p25 is an occlusion-derived virus envelope protein (ID# AAA66724.1). MKBAC-65.2 has been validated for Western blots of infected Sf9 cell lysates, immunoprecipitation of p25 from infected Sf9 cells and staining of infected cells. Additional data provided upon request.
Hamster monoclonal antibody against Nedd4 family interacting protein-1 (Ndfip1)

Kappler; Marrack

Ndfip1 is a protein binding to Nedd4, a HECT-type 3 ubiquiting ligase. This hamster monoclonal antibody reacts to the immunogen by ELISA assay and can be used in Western blot and immunohistochemistry analysis.  Additional data provided upon request.

Publication abstract

Mouse monoclonal antibody against bacterial Sigma 54 RNA polymerase

Kappler; Marrack

This mouse monoclonal antibody reacts to the immunogen by ELISA assay and can be used in Western blot analysis.  Additional data provided upon request.
Hamster monoclonal antibody against mouse Spi2A (Serine protease inhibitor 2A)

Kappler; Marrack

This hamster monoclonal antibody reacts to the immunogen by ELISA assay and can be used in Western blot analysis.  Additional data provided upon request.
Mouse monoclonal antibody against the acid/base leucine zipper

Kappler; Marrack

This mouse monoclonal antibody reacts to the immunogen by ELISA assay and can be used in Western blot analysis.  Additional data provided upon request.
Mouse monoclonal antibody against the phage protein pIV

Kappler; Marrack

This mouse monoclonal antibody reacts to the immunogen by ELISA assay and can be used in Western blot analysis.  Additional data provided upon request
CD8-transfected BW5147 alpha-beta- cell line/fusion partner (BWlyt2-4)

Kappler; Marrack

Useful research tool for the study of antigen processing and antigen presentation requirements of class I-restricted T cells

Publication abstract

Hamster monoclonal Ab anti mouse Bcl-3 (Ham150-3.5)

Kappler; Marrack

This Hamster monoclonal antibody anti mouse Bcl-3 is a IgG1 Lambda. The antibody reacts to the immunogen by ELISA assay. Western blot analysis shows a band at the right molecular weight in: 1) normal mouse cells 2) mouse cells overexpressing Bcl-3. In this case, the band does show a substantial increase in Bcl-3 3) the human T cell line Jurkat.
DO-11.10 T cell hybridoma  

Kappler; Marrack

The hybridoma D0-11.10, a mouse monoclonal antibody against receptors for antigen plus I region products on T cells.  Use of I region-restricted, antigen-specific T cell hybridomas to produce idiotypically specific anti-receptor antibodies.

Publication abstract

BW5147 alpha-beta- cell line/fusion partner

Born; Kappler; Marrack

A variant of the AKR thymona BW5147 has been isolated which can no longer express functional T cell receptor alpha- and beta- chains.

Publication abstra ct

4 monoclonal antibodies anti-staphylococcal enterotoxin B

Kappler; Marrack

2 antibodies (B344 and B327) bind to epitopes not required for superantigen function, 1 antibody (2B33) blocks an MHC interaction site on the staphylococcal enterotoxin B (SEB) and 1 antibody (B87) blocks the T cell recognition site on SEB.

Publication abstract

Four mouse monoclonal anitbodies specific for SEA

Kappler; Marrack

Clone A108 crossreacts with SEE, overlaps Zn++ MHC binding site on SEA, and requires Zn++ for binding.  Clone A111 and A228 crossreact with SEE.  Clone A116 does not crossreact with SEE and recognizes a separate epitope than A108.
A116 and A108 were used in the study described in this Publication abstract
One mouse Mab specific for SEC

Kappler; Marrack

Clone C165 reacts with SEC1 (1.5nM) > SEC3 (20nM) > SEC2 (75nM)
KMAC-92.6 and KH-2 T cell hybridomas

Kappler; Marrack

Publication abstract
Rat monoclonal Ab anti mouse T cell receptor Vb8.1 + 8.2 (KJ16)

Kappler; Marrack

Publication abstract
Hamster monoclonal Ab anti human CD3 (H25-1157)

Kubo

Epitope is expressed by both CD3 gamma and delta.  mAb reactivity tested by immunoprecipitation and western blotting. mAB does not react with surface expressed CD3. Cross-reactivity shown with mouse CD3.  Publication abstract
Hamster monoclonal Ab anti human CD3 epsilon (H25-1352)

Kubo

mAb reactivity tested by immunoprecipitation and western blotting. mAb does not react with surface expressed CD3. Cross reactivity shown with mouse, rat, sheep, bovine and porcine CD3.

Publication abstract

Hamster monoclonal antibody anti mouse T cell receptor a b -pan-reactive (designated H57-597).

Kubo

Hamster monoclonal antibody (H57-597) anti mouse T cell receptor alpha beta-pan-reactive. Epitope expressed by Cbeta. mAb reactivity tested by IF staining, stimulation of T cells and immunoprecipitation. Publication abstract
Hamster monoclonal Ab anti-mouse T cell receptor -alpha/beta (H28-710)

Kubo

Reactivity by immunoprecipitation and western blotting mAB does not react with live T cells and does not induce T cell activation. Publication abstract
Mouse hybridomas expressing gamma delta T cell receptors

BornO'Brien

Hybridomas expressing various combinations of gamma delta T cell receptors available as research tools.  Publication abstract
Hamster monoclonal Ab anti mouse CD3 zeta chain (H146-968)

Kubo

Publication abstract
5KC73 and 3QO23-24 T cell hybridomas  

Kappler; Marrack

Two T cell hybridomas (5KC-73 & 3QO23-24) bearing Vbeta3 TCR or Vbeta17 TCR. The hybridoma D0-11.10, a mouse monoclonal antibody against receptors for antigen plus I region products on T cells.  Use of I region-restricted, antigen-specific T cell hybridomas to produce idiotypically specific anti-receptor antibodies.  Publication abstract
Mouse monoclonal antibody against human Vbeta13.1 or Vbeta13.2 T cell receptor

Kappler; Marrack

Publication abstract
BALB/c-derived, cOVA-specific, Id-restricted T cell hybridomas

Kappler; Marrack

Publication abstract
For more information about research tools, contact Emmanuel Hilaire at hilairee@njc.org

© Copyright 2008 National Jewish Health