Mouse Genetics

The Mouse Genetics Core Facility provides investigators the possibility of generating genetically engineered mice by transgenic and gene targeting methodologies. The core can generate, genetically manipulate, and select mouse embryonic stem (ES) cells from various genetic strains for injection into blastocysts. Alternatively, these genetically modified ES cells can be differentiated in vitro by the core facility into various cell types for investigator research. The core also offers injection of transgenes into fertilized oocytes.

The facility can advise investigators on the generation of transgenic and targeting constructs for simple knock-out/knock-in and for tissue-specific and conditional mutations as well as strategies for screening transfected ES cells and mouse mutants. In addition, the core facility performs rederivation of "dirty" mice by embryo transfer and cryopreservation of mouse strains (embryos).

Our Services

• Consultation on design of transgene and gene targeting vectors and genetic screening
• Gene targeting in ES cells
• ES cell injection into blastocysts and generation of chimeric mice
• In vitro ES cell differentiation
• DNA microinjection and generation of transgenic mice
• Strain rederivation and resuscitation from living or frozen embryos
• Strain cryopreservation