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Cytometry

National Jewish Health
K1014 Goodman Building
303.398.1330
Fax: 303.398.1225
loomisj@njhealth.org
sobuss@njhealth.org
More info: http://flowlab.njc.org/

Instrument	Main Uses
Analyzers:
LSR	II 355nm 405nm 488nm 532nm 635nm lasers	Up to 18 color analysis
CYAN	405nm 488nm 630 lasers	Up to 9 color analysis
FACScan	488nm 630nm lasers	Up to 5 color analysis
FACScalibur	488nm 640 lasers	Up to 4 color analysis
Accuri	488nm 635nm lasers	Up to 4 color analysis
IS 100	488nm laser	Flow imaging

Sorters
Synergy	355nm 488nm 640nm 405nm 561nm lasers	Up to12 color sort & analysis
MoFlo XDP	488nm 405nm 635nm lasers	Up to 9 color sort & analysis

Microscopes
IP Lab	Long working distance fluorescence
Leica	4 color slides, DIC, Z
LWD Marianas	Long working distance, dry objectives
Marianas	Multicolor, Z, live cell, Ca++ flux
Olympus	Rapid multicolor scope for live cell
Olympus/Apogee	Brightfield absorption stained slides
Smith Marianas	Inverted multicolor, Z
Zeiss LSM 700 Confocal	Multicolor, Z, time, colocalization
MultiPhoton Olympus	Deep tissue, in situ live tissue imaging

Programs for computational data analysis
AxioVision	Zeiss analysis software for widefield system
Cell Quest	FCS data on MAC
FloJo	FCS on MAC
Ideas	Imagestream analysis
Image J	NIH image analysis IBM
Iplab	Microscope data MAC
LSM 510	Zeiss confocal analysis
NIH Image	Microscope data MAC
Real-14	Microscope data IBM
Slidebook	Microscope data MAC + IBM
Zen	Zeiss confocal analysis
Imaris	Microscope data Multiphoton and Zeiss Confocal