Cytometry: Instruments and Services

National Jewish Health
K1014 Goodman Building
303-398-1330 
Fax: 303-398-1225
veltrig@njhealth.org
More info: http://flowlab.njc.org/

Instrument

Main Uses

CYAN 405nm, 488 , 630nm lasers rapid 9 color fluorescence
FACSscan with Rainbow 488, 630 up to 5 color analysis
FACScalibur with 640 nm excitation up to 4 color analysis
BD LSR  Argon, HeNe, HeCd up to six colors [Ca++] flux
MoFlo with visable,uv,  and  633nm rapid cell sorting, up to eight color
MoFloII with visable, and  633nm rapid cell sorting, up to 4 color
Imagestream 100 flow imaging
Signal Analytics IP Lab imaging longworking distance fluorescence
Leica-Slidebook scope 4 color slides and DIC images, z
Marianas Slidebook scope multicolor,z,live cell,Ca++ flux
Neustadt slidebook inverted multicolor, z sectioning
T.I.L.L. Olympus rapid multicolor scope for live cell imaging
Long working distance Marianas long working distance dry objectives
Olympus/Apogee brightfield absorption stained slides
Zeiss confocal/multiphoton deep tissue imaging, FRAP
 

Analysis Available on Computer Systems

TILLvisION TILL data
ImageJ NIH image analysis program IBM
Cell Quest FCS data on a MAC
Mcycle DNA data on a IBM
Modfit DNA data on a MAC
Mtime time course experiments like [Ca++]
Repromac FCS data on MAC for extra graphics applications
NIH Image microscope data on MAC
Real-14 microscope data on IBM
Iplab microscope data on MAC
Slidebook microscope data on MAC + IBM
FloJo flow data on MAC
IDEAS Imagestream analysis
LSM 510 Zeiss confocal analysis software


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