Pathology and Electron Microscopy
The Pathology and Electron Microscopy Laboratory is a Morphology Core Facility, which provides histopathological, immunocytochemical, and ultrastructural support for clinicians and research investigators at National Jewish Health, several investigators at The Children's Hospital and University of Colorado Denver.
The facility provides quality control for morphologic studies, and optimizes use of supplies and equipment. Ongoing interaction of our personnel with each investigator facilitates communication regarding morphologic findings, histopathological interpretation, and new technical developments, thus increasing the efficiency of the research projects.
Our personnel are experienced, dedicated technicians who are accustomed to serving specialized clinical and research needs. A full range of histologic routine and special staining procedures are available. Immunohistochemistry and electron microscopy techniques are also offered. Expert advice is available regarding optimal staining methods for special projects.
Areas of Research and Clinical Pathology
Our areas of expertise include, but are not limited to:
- Clinical Pathology and Diagnostic Electron Microscopy
Evaluation of bronchial biopsies, nasal biopsies and intestinal tract samples for light level abnormalities. Electron microscopic evaluation of bronchial and nasal biopsy samples for abnormalities of ciliary function and for the presence of atypicial infections.
Animal models of inflammation and pathologic states and in human pulmonary diseases.
We have extensive experience in the evaluation of development and resolution of pulmonary inflammation, fibrosis, and in animal "models" of asthma. In the past ten years we have also explored the involvement of chronic infection with atypical pathogens in the persistence of asthma. We have a long-standing interest in pulmonary vascular remodeling in pulmonary hypertension.
Experimental responses of macrophages and neutrophils to mediators of inflammation. Apoptosis (studies in neutrophils, lymphocytes and epithelial cells).
Major Capital Equipment
- Philips 400 T Electron Microscope
- Philips CM 10 Electron Microscope
- Reichert- Jung Ultracut E ultramicrotomes
- Lynx EM Tissue Processor
- Miles-Sakura VIP Tissue processor
- Miles-Sakura Embedding Center
- Reichert -Jung Cryocut Cryostat
- Zeiss Light Microscope
- Olympus Double-headed Light Microscope Equipped with Camera
- Olympus Triple-headed Microscope
- Morphometry Equipment
- Omega Enlarger
- IEC Centrifuge
- Vacuum Pumps
- Mettler PC 440 Balance
- JB -4 Microtome
- Lab Line Incubator/oven
- Beckman Microfuge
- Mohr Print Processor
Pathology and Electron Microscopy Laboratory
Hanley Building, Room F111
National Jewish Health
1400 Jackson St.
Denver, CO 80206